三子养亲汤、桃红四物汤单用及联用治疗哮喘大鼠作用的比较  

Comparison of Therapeutic Effect of Sanzi Yangqin Decoction, Taohong Siwu Decoction and Their Combination for Asthmatic Rats

作　　者：黄丰[1] 吴赛春[1] 李满萍[1] 涂苑青[1] 曾静[1] 童晓云[2] 李晓[2] 

机构地区：[1]暨南大学药学院中药药理教研室,广东广州510632 [2]云南中医学院第一附属医院老年病科一病区,云南昆明650021

出　　处：《中药新药与临床药理》2013年第5期457-461,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81073129)

摘　　要：目的比较三子养亲汤、桃红四物汤单用及联用对哮喘大鼠的作用效果。方法 SD大鼠以卵清白蛋白(OVA)致敏激发复制模型,并予药物进行干预。采用ELISA法检测肺泡灌洗液(BALF)中γ干扰素(IFN-γ)、白介素4(IL-4)含量,并用Western Blot法检测肺组织中磷酸化p38蛋白激酶(p38 MAPK)的表达水平。结果与正常对照组比较,模型组大鼠BALF中IL-4含量明显升高(P<0.01),IFN-γ含量明显降低(P<0.01),IFN-γ/IL-4比值下降(P<0.01);与模型组比较,各药物组IL-4水平下降、IFN-γ水平提高,IFN-γ/IL-4比值升高,差异均有统计学意义(P<0.05,P<0.01);化痰活血方组与三子养亲汤及桃红四物汤组比较,差异均有统计学意义(P<0.05,P<0.01)。模型组大鼠肺组织磷酸化p38 MAPK表达较正常对照组明显增加(P<0.01),各药物组磷酸化p38 MAPK表达下降,且化痰活血方组与三子养亲汤组、桃红四物汤组比较,差异均有统计学意义(P<0.05,P<0.01)。结论三子养亲汤、桃红四物汤联用组成的化痰活血方对哮喘大鼠的治疗作用优于单用,可能与抑制p38 MAPK磷酸化,调节Th1/Th2平衡有关。Objective To compare the therapeutic effect of separate use or combined use of Sanzi Yangqin Decoction （SYD） and Taohong Siwu Decoction （TSD） for asthmatic rats. Methods SD rats were sensitized and challenged by OVA to build asthmatic model, and then the model rats were interfered orally with SYD, TSD or SYD-TSD combination （Huatan Huoxue recipe）. The levels of interferon-gamma （IFN-7） and interleukin-4 （IL-4） in bronchoalveolar lavage fluids（BALF） were detected by enzyme-linked immunosorbent assay（ELISA）. The expression of phosphorylation-p38 mitogen-activated protein kinase （MAPK） in the lung tissue was detected by Western blotting method. Results In the model group, the level of IFN- γ was lower but the level of IL-4 was higher in BALF than that in the normal control group, and the IFN-γ/IL-4 ratio was also lower（P 〈 0.01 ）. SYD, TSD or SYD-TSD combination could all significantly reduce the level of IL-4, and enhance IFN-γ/ level and IFN-γ/IL-4 ratio in BALF of asthmatic rats（P 〈 0.01 compared with those in the model group） , and the effect in SYD-TSD combination group differed from that of SYD group and TSD group （P 〈 0.05 or P 〈 0.01 ）. The phosphorylation-p38 MAPK in the model group was highly expressed when compared to normal control group（P〈 0.01 ）, and the high phosphorylation- p38 MAPK expression was suppressed by all of the medicine, the effect in SYD-TSD combination group being stronger than that of SYD group and TSD group（P 〈 0.05 or P 〈 0.01 ）. Conclusion The combined use of SYD and TSD has better effect than separate use in treating asthmatic rats, and the mechanism might be related with inhibiting phosphorylation-p38 MAPK expression and with the regulating the balance of Thl and Th2.

关 键 词：化痰活血方 哮喘 白介素4 Γ干扰素 P38蛋白激酶 

分 类 号：R285.5[医药卫生—中药学]