喘平方及其拆方的平喘作用  

Experimental Study of Chuanping Recipe and Its Separate Recipe in Calming Panting

作　　者：张雄飞[1] 黄娟萍 罗裕[1] 刘静[1] 梁锦杰 朱盛山[1] 

机构地区：[1]广东药学院中药开发研究所,广东广州510006 [2]广州普平生物科技有限公司,广东广州510000

出　　处：《中药新药与临床药理》2013年第5期461-465,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家"十二五"重大新药创制科技重大专项(2011ZX09102-011-01)

摘　　要：目的阐明喘平方组方的合理性,为临床使用该方提供实验依据。方法采用乙酰胆碱-组胺喷雾致喘法,观察喘平方及其拆方对豚鼠哮喘潜伏期的影响;体外实验观察喘平方及其拆方对组胺致豚鼠离体气管平滑肌收缩松弛作用的影响。结果喘平方及其拆方对组胺-乙酰胆碱诱发的喘息有保护作用,对组胺致豚鼠气管平滑肌痉挛有松弛作用。与方中各药材分别单提后再按处方配比混合组成的复方比较,喘平复方合煎组平喘作用无明显差别,但较方中各主药分别单提作用强;同时比相当于单个给药剂量喘平方提取液中所含已知主要活性成分麻黄碱、东莨菪碱的量的单体混合后给药平喘作用强。结论喘平方有较强的平喘作用,其组方具有合理性与优越性;中药复方作用并非仅仅由方中几个已知主要活性成分提供。Objective To elucidate the rationality of the combination of Chuanping Recipe, and to provide the experimental basis for the clinical use of this Chinese herbal formula. Methods Guinea pigs models of allergic asthma induced by acetylcholine-histamine were used to observe the effect of Chuanping Recipe and its separate recipe on asthma latency in the guinea pigs, and in-vitro experiment was carried out for the observation of their effect on the isolated tracheal smooth muscle of histamine-induced asthma guinea pigs. Results Chuanping Recipe and its separate recipe could notably prolong the asthma incubation period in allergic asthma guinea pigs induced by acetylcholine-histamine, and could relax the spasm of isolated tracheal smooth muscle of histamine-induced asthma guinea pigs. There was no significant difference of the anti-asthmatic effect between the decoction of Chuanping Recipe and the extracts of the single herb. The decoction of Chuanpiug Recipe showed stronger anti-asthmatic effect than the decoction of extracts of the single principal herb and also than the mixture of ephedrine and pseudoephedrine extracted from Chuanping Recipe. Conclusion Chuanping Recipe shows stronger anti-asthmatic effect than the known main active compositions, indicating the rationality and advantage of the formula.

关 键 词：喘平方 哮喘 平喘作用 豚鼠 

分 类 号：R285.5[医药卫生—中药学]