杏香兔耳风总酚酸耐药突变选择窗的体外研究  

In-vitro Studies on Mutant Selection Window of Total Phenolic Acids from Ainsliaea fragrans Champ.

作　　者：刘晓[1] 苏丹[1,2] 谢斌[1] 张武岗[1,2] 冯育林[1,2] 杨世林[1,2] 

机构地区：[1]江西中医药大学,江西南昌330006 [2]中药固体制剂制造技术国家工程研究中心,江西南昌330006

出　　处：《中药新药与临床药理》2013年第5期465-468,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然青年科学基金项目(81102787);国家"十一五"科技支撑计划(2006BAI06A01-01);国家"重大新药创制"科技重大专项(2009ZX09103-352);江西省卫生厅中医药科研计划(2012A158);江西省自然科学基金重点项目(20133ACB21005)

摘　　要：目的测定杏香兔耳风总酚酸和对照药绿原酸对金黄色葡萄球菌ATCC26003和大肠埃希菌ATCC44102的防耐药变异浓度(MPC)。方法采用琼脂平板二倍稀释法,测定杏香兔耳风总酚酸和绿原酸对金黄色葡萄球菌和大肠埃希菌的最小抑菌浓度(MIC)、99%抑菌浓度(MIC99)、初测MPC(MPCpr)和MPC。结果杏香兔耳风总酚酸、绿原酸对金黄色葡萄球菌的MIC、MPC和细菌耐药选择指数(MPC/MIC99)分别为0.4,6.0 mg·mL-1;10.8,8.4 mg·mL-1;34.0,1.6。对大肠埃希菌的MIC、MPC和MPC/MIC99分别为12.0,6.0 mg·mL-1;16.8,9.6mg·mL-1;1.4,1.7。结论杏香兔耳风总酚酸限制金黄色葡萄球菌耐药突变菌株作用强于绿原酸,限制大肠埃希菌耐药突变菌株作用弱于绿原酸。Objective To measure the mutation preventing concentration（MPC） of total phenolic acids from A insliaea fragrans Champ. and control drug of chlorogenie acid for Staphylococcus aureus ATCC26003 and Escherichia coli ATCC44102. Methods The minimal inhibitory concentration （MIC）, MIC for 99 % of input cells （MIC99）, provisional MPC （MPCpr） and MPC of total phenolic acids from Ainsliaea fragrans and chlorogenic acid for Staphylococcus aureus and Escherichia coli were determined by agar plate doubling dilution method. Results The MIC, MPC and MPC/MIC99 of total phenolic acids from A insliaeafragrans for Staphylococcus aureus strain ATCC26003 were 0.34mg·mL^-1, 10.8mg·mL^-1, 34.0, and those of chlorogenie acid were 6.0 mg＇mL-1, 8.4mg·mL^-1, 1.6, respectively. The MIC, MPC and MPC/MIC99 of total phenolic acids for Escherichia coli strain ATCC44102 were 12.0 mg·mL^-1, 16.8mg·mL^-1, 1.43, and those of chlorogenic acid were 6.0mg·mL^-1, 9.6 mg·mL^-1, 1.7, respectively. Conclusion The effect of Ainsliaea fragrans total phenolic acids is stronger for restricting Staphylococcus aureus resistant mutants while is weaker for restricting Escherichia coli resistant mutants than that of chlorogenic acid.

关 键 词：杏香兔耳风总酚酸 金黄色葡萄球菌 大肠埃希菌 防耐药变异浓度 

分 类 号：R285.5[医药卫生—中药学]