白英甾体总生物碱对人肺癌细胞A549裸鼠异种移植瘤的药效研究  

In-vivo Inhibiting Activity of Total Steroidal Alkaloids from Solanum lyrantum Thunb. on A549 Lung Cancer Malignant Cell Lines in Nude Mice

作　　者：王建农[1] 臧雅丽[1] 顾士萍[1] 卜璟[1] 

机构地区：[1]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中药新药与临床药理》2013年第5期469-472,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家重大新药创制(2009ZX09103-402);北京市自然科学基金(7102137)

摘　　要：目的探讨白英抗肿瘤有效部位对人肺癌细胞A549裸鼠异种移植肿瘤生长抑制作用及作用强度。方法在裸鼠腹腔内接种人肺癌细胞A549细胞株复制肿瘤模型,给予阳性对照药紫杉醇和白英甾体总生物碱干预后,测量肿瘤体积和肿瘤质量,计算肿瘤生长抑制率,比较组间抗肿瘤药效强度。结果白英低、中、高剂量组(12,24,48 mg·kg-1)的相对肿瘤增殖率(T/C)分别为85.52%、60.64%、45.24%,抑瘤率分别为2.35%、49.79%、48.93%,而相同条件下,紫杉醇组(8 mg·kg-1)的T/C和抑瘤率分别为14.68%、82.59%。紫杉醇有明显的抗肿瘤作用,白英甾体总生物碱均有一定的抗肿瘤作用,其中中、高剂量组抗肿瘤作用更明显。结论白英甾体总生物碱对人肺癌细胞A549裸鼠异种移植肿瘤的生长具有抑制作用。Objective To study the inhibitory effect of active fractions of Solarium lyrantum Thunb. on A549 lung cancer malignant cell lines in nude mice. Methods Three different dosages of Solanum lyrantum total steroidal alkaloids solution and positive drug of paclitaxel solution were given to the nude mice which were inoculated with A549 lung cancer cells intraperitoneally. At the end of drug intervention, tumor mass size and tumor mass weight were measured, tumor-inhibitory rate was evaluated, and the anti-tumor effect of different groups was compared by SPSS software. Results The relative tumor proliferation rate（T/C） was 85.52 %, 60.64 %, and 45.24 % in 12, 24, 48mg·kg^-1 total steroidal alkaloids groups respectively, and tumor-inhibitory rate was 2.35 %, 49.79 %, and 48.93 % accordingly. Under the same condition, T/C was 14.68 % and tumor-inhibitory rate was 82.59 % in paclitaxel （8 mg·kg^-1） positive control group. Conclusion Total steroidal alkaloids from Solanum lyrantum Thunb. shows certain tumor-inhibitory effect on A549 lung cancer cells in nude mice.

关 键 词：白英 抗肿瘤 甾体总生物碱 A549肺癌细胞株 

分 类 号：R285.5[医药卫生—中药学]