近红外光谱法快速分析广升麻药材  

Rapid Analysis of Radix Serratulae Chinensis by Near-Infrared Spectroscopy

作　　者：肖浩[1] 严小红[2] 江英桥[2] 王自[2] 侯惠婵[2] 

机构地区：[1]广州中医药大学,广东广州510006 [2]广州市药品检验所,广东广州510160

出　　处：《中药新药与临床药理》2013年第5期506-509,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家药品标准提高暨2015版药典科研项目

摘　　要：目的建立广升麻药材快速分析方法。方法建立广升麻药材一致性模型与聚类分析模型,运用最小偏二乘法(PLS)建立广升麻中蜕皮甾酮含量与其近红外光谱之间的校正模型,并对未知样品进行含量预测。结果利用一致性模型与聚类分析模型能够准确的区分广升麻药材与其混用品,定量校正模型决定系数R2为92.34,内部交叉验证均方差(RMSECV)为0.94,外部验证均方差(RMSEP)为0.33。结论该方法准确、快速,为广升麻药材的快速分析方法的建立提供了科学依据。Objective To develop a method for rapid analysis of Radix Serratulae Chinensis. Methods We established conformity model and cluster analysis model for Radix Serratulae Chinensis, and partial least squares were used to establish the calibration model for content of ecdysone in Radix Serratulae Chinensis and near-infrared spectrum. Results The conformity and cluster analysis model can be used to accurately identify Radix Serratulae Chinensis and its adulterants, the determination coefficient of calibration was 92.34, root mean square error of cross validation（RMSECV） was 0.94, and root mean square error of prediction deviation（RMSEP） was 0.33. Conclusion The near-infrared spectroscopy method is accurate and rapid, which provides the scientific basis for the rapid analysis of Radix Serratulae Chinensis.

关 键 词：近红外光谱法 广升麻 快速分析 

分 类 号：R284.1[医药卫生—中药学]