戊己丸不同配伍方对结肠平滑肌细胞5-HT3,4受体及下游信号传导通路主要元件的影响  

Effects of Wuji pill in different compatibilities on colon smooth muscle cells5-hydroxytryptamine 3,main element 4 receptor and downstream signal transduction pathway

作　　者：王迎寒[1,2] 周淑媛[1] 巩仔鹏[1] 杨庆[1] 王娅杰[1] 朱晓新[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]承德医学院,河北承德067000

出　　处：《中国中医基础医学杂志》2013年第9期783-786,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金重点资助项目(30930114);国家国际科技合作项目(S2011ZR0193)

摘　　要：目的:观察戊己丸不同配伍方对平滑肌细胞5-羟色胺3,4受体及其下游信号通路的影响。方法:采用Bitar的方法分离培养大鼠结肠平滑肌细胞,α-actin免疫组化鉴定。应用免疫荧光法和钙离子探针检测5-羟色胺3,4受体表达及Ca2+浓度;应用ELISA试剂盒检测cAMP,PKA的浓度。结果:1号方和2号方对大鼠结肠平滑肌细胞5-羟色胺3,4受体的表达及Ca2+浓度均具有抑制作用(P<0.05);可显著下调平滑肌细胞的cAMP和PKA水平(P<0.05);2号方作用效果优于1号方。结论:戊己丸可抑制5-羟色胺3,4受体表达及下调Ca2+,cAMP,PKA水平。Objective：To study the influence of Wuji pill on 5- serotonin（5-HT） downstream signal pathway. Methods： According to Bitar＇ s methods, the rat colon smooth muscle cells （SMC） were isolated and cultured, and then identified by α-actin immunohistochemistry stain. Furthermore, the 5-HT receptor 3, 4 of the SMC membrane and Ca2＋were tested by immunofluorescence stain and calcium ion probe. The cyclic adenosine monophosphate （cAMP） and protein kinase A （PKA） level were measured by ELISA. Results：Two compatibilities can decrease the expression of 5-HT receptor 3,4 and the concentration of Ca2＋ of the SMC significantly（ P 〈 0.05 ） , furthermore down-regulate the level of cAMP and PKA of the SMC significantly（P 〈 0.05）. The effect of NO. 2 Wuji pill was better than No. 1. Conclusion ： Wuji pill can decrease the expression of 5- serotonin receptor 3,4 and down-regulate the level of Ca^2＋ ,cAMP and PKA.

关 键 词：戊己丸 大鼠结肠平滑肌细胞 5-羟色胺3受体 5-羟色胺4受体 环磷酸腺苷 

分 类 号：R285.5[医药卫生—中药学]