缺血后处理对肾缺血/再灌注损伤后内皮型一氧化氮合酶表达的影响  

Effects of Ischemic Postconditioning on eNOS Expression in Rats with Renal Ischemia/Reperfusion Injury

作　　者：陈晖[1] 刘修恒[1] 王磊[1] 翁小东[1] 邱涛[1] 刘林[1] 

机构地区：[1]武汉大学人民医院泌尿外科,武汉430060

出　　处：《医学综述》2013年第18期3372-3376,共5页Medical Recapitulate

基　　金：国家自然科学基金(30901494)

摘　　要：目的观察缺血后处理对大鼠急性肾缺血/再灌注损伤的保护作用及缺血后处理对内皮型一氧化氮合酶(eNOS)表达的影响。方法将2012年9月购自武汉大学动物实验中心的38只8周龄雄性Wistar大鼠依据随机数字表法分为4组:假手术组(8只)、缺血/再灌注组(10只)、缺血后处理组(10只)、缺血预处理组(10只)。建立原位大鼠单侧肾缺血/再灌注动物模型,摘除右肾后对左肾行缺血后处理,10 s再灌注,10 s缺血,6次循环后再灌注24 h。采用全自动生化分析仪检测血尿素氮、肌酐,比色法测定血浆一氧化氮。免疫组织化学法和反转录聚合酶链反应(RT-PCR)检测eNOS表达。蛋白免疫印迹法(Western blot)检测胞质eNOS水平。结果肾缺血/再灌注24 h后,缺血/再灌注组中血尿素氮、肌酐、一氧化氮较假手术组显著增高(P<0.05),组织中eNOS表达较假手术组升高(P<0.05),缺血后处理组和缺血预处理组中的血尿素氮、肌酐较缺血/再灌注组降低(P<0.05),血清一氧化氮和组织中eNOS较缺血/再灌注组中升高更显著(P<0.05)。结论缺血后处理对大鼠肾缺血/再灌注损伤有保护作用,具有临床应用价值。其保护机制可能与缺血后处理诱导eNOS的合成增多有关。Objective To investigate the protective effect of ischemic postconditioning on rats with acute renal ischemia/reperfusion（I/R） injury and its effect on endothelial nitric oxide synthase（eNOS） and nitric oxide（NO）. Methods A rat I/R model was established with 38 male Wistar rats aged 8 weeks purchased from Wuhan University Animal Experiment Center in September 2012, which were divided into 4 groups according to random number table method：sham group （8）, ischemia/reperfusion group （lO） , postcondition- i ng ischemia group （ 10 ） , preconditioning ischemia group （10）. The renal postconditioning was induced by 6 cycles of left renal reperfusion （ 10 s ） and ischemia （ 10 s ） followed by reperfusion （24 h ） after resection of right kidney. The parameters of renal function were determined by auto-biochemical analyzer. The concentra-tions of NO were measured with chromometry,eNOS in cytoplasm was examined by immunohistochemistry, RT-PCR and Western blot. Results After ischemia and reperfusion injury for 24 h,compared with the sham group, BUN, Cr and NO increased significantly in the rats undergoing I/R procedure （ P 〈 0.05 ）. The release of eNOS increased significantly in the rats undergoing L/R procedure compared with the sham group （ P 〈 0.05 ）. After ischemic postcondtioning and ischemie preconditioning, the levels of BUN, Cr were signifi- cantly decreased （ P 〈 0. 05 ）, NO in the serum and the release of eNOS were significantly increased （P 〈0.05）. Conclusion Isehemie postcondtioning can protect the rats subjected to ischemia and reperfu-sion injury and has the value of clinical application. Its protective mechanism may be related to the increase of the release of eNOS induced by ischemie postconditioning.

关 键 词：缺血后处理 缺血 再灌注 内皮型一氧化氮合酶 一氧化氮 

分 类 号：R332[医药卫生—人体生理学] R619.9[医药卫生—基础医学]