复方血栓通胶囊中4个有效成分的一测多评定量方法研究  

A Quantitative Method Using One Marker for Simultaneous Assay of Four Effective Components in Compound Xueshuantong Capsule

作　　者：梁洁萍[1] 陈思[1] 谢称石 彭维[1] 苏薇薇[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广东众生药业股份有限公司,广东东莞523325

出　　处：《中山大学学报（自然科学版）》2013年第5期123-126,129,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家"重大新药创制"科技重大专项资助项目(2011ZX09201-201-22);国家科技支撑计划课题(2012BAI29B09);东莞医疗卫生科技计划资助项目(2012105102004)

摘　　要：为建立复方血栓通胶囊中4个有效成分的一测多评定量方法,采用高效液相色谱法,以人参皂苷Rg1为参照对照品,计算其与三七皂苷R1、人参皂苷Re、人参皂苷Rb1的相对校正因子,并进行含量计算,实现一测多评;同时采用外标法验证一测多评法的准确性。实验结果显示校正因子重现性好,采用校正因子计算的含量值和外标法实测值之间没有显著性差异。故采用一测多评法同时测定复方血栓通胶囊中4个有效成分的含量,具有准确、简便、经济实用等优点,值得推广。The paper reported the establishment of a quantitative method for simultaneously determining four effective components in Compound Xueshuantong capsule by using one chemical reference, the new quality evaluation method, quantitative analysis of multi-components by single marker （ QAMS）, using Ginsenoside RgI as the internal reference substance, was developed by HPLC to simultaneously determine Notoginsenoside R1, Ginsenoside Rgl, Ginsenoside Re, Ginsenoside Rbl in Compound Xueshuantong capsule with the relative correction factor （RCF）. In addition, the results of QAMS method were valida- ted by comparing with that of external standard method. The results show that the RCFs have good repro- ducibility and no significant differences between the quantitative results of QAMS method and external standard method were observed. So, it is a accurate, convenient and practical method to simultaneously determine four effective components in Compound Xueshuantong capsule for its quality control.

关 键 词：复方血栓通胶囊 一测多评 校正因子 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]