枫杨组织培养快繁技术研究  

Study on Rapid Micropropagation System of Pterocarya stenoptera C.DC. by Tissue Culture

作　　者：王春荣[1,2] 毕君[1,2] 张往祥[3] 曹福亮[3] 

机构地区：[1]河北省林业科学研究院,石家庄050061 [2]河北省林木良种工程技术研究中心,石家庄050061 [3]南京林业大学,南京210037

出　　处：《中国农学通报》2013年第25期42-48,共7页Chinese Agricultural Science Bulletin

基　　金：国家科技部"十二五"农村领域国家科技计划课题"逆境生态林树木种质优选与示范"(2011BAD38B01)

摘　　要：为了实现枫杨优良种源和变异个体的种质保存和快速繁殖,选用枫杨带腋芽半木质化茎段为外植体,进行组织培养技术研究。结果表明：枫杨外植体适宜消毒处理为75%酒精30 s＋0.1%HgCl25 min,外植体接种成功率可达87.5%;枫杨愈伤增殖基本培养基以MS为宜,而试管芽苗增殖和根诱导DKW培养基明显优于MS、B5;试管芽苗增殖适宜培养基为（1.0~2.0）mg/L 6-BA＋1.0 mg/L KT＋（0.3~0.5）mg/L IBA,20~25天增殖4倍以上;根诱导以1/2 DKW＋（0.5~0.7）mg/L NAA和1/2 DKW＋0.5 mg/L NAA＋0.2 mg/L IAA为宜,生根率82%以上。For germplasm conservation and rapid propagation of Pterocarya stenoptera C.DC., the stem-segment with axillary buds taken as explants to be studied for tissue culture system. The results showed that, the best sterilization method was that explants could be treated with 75% ethyl alcohol and 0.1% HgCl2 for 30 s and 5 min, respectively. The percentage of sterile explant could arrive at 87.5%. The best basic medium of callus proliferation was MS, but DKW was better than MS and B5 to be used in test-tube plantlet multiplication and root induction. It was suggested that DKW supplemented with （1.0-2.0） mg/L 6-BA ＋ 1.0 mg/L KT＋（0.3-0.5） mg/L IBA to be desirable treatment for subculture and multiplication, with 4 times multiplication rate after a 20-25 days cultivation. The media with 1/2 DKW＋（0.5-0.7） mg/L NAA or 1/2 DKW＋ 0.5 mg/L NAA＋0.2 mg/L IAA was suggested for the rooting induction. The rooting rate was above 82%.

关 键 词：枫杨 组织培养 枝段 快繁 根诱导 

分 类 号：S723.1[农业科学—林木遗传育种]