应用RNAi技术获得抗ToMV和CMV转基因烟草  

Transgenic Tobacco Resistant to ToMV and CMV Obtained by RNA Interference

作　　者：林涛[1] 郑银英[1] 崔百明[1] 乔亚红[1] 向本春[1] 

机构地区：[1]石河子大学生命科学学院/石河子大学农业生物技术重点实验室,石河子832003

出　　处：《石河子大学学报（自然科学版）》2013年第4期430-435,共6页Journal of Shihezi University(Natural Science)

基　　金：国际科技合作与交流专项(20072072)

摘　　要：为了获得同时抗ToMV和CMV的转基因烟草,采用以ToMV和CMV部分CP基因片段作为干扰病毒的靶序列,构建了pBi35SG12RNAi表达载体。利用农杆菌介导法转化西方烟(Nicotiana occidentalis),经卡那霉素筛选、PCR鉴定证明干扰序列已整合到烟草基因组中,并对ToMV和CMV混合侵染表现抗性。To obtain transgenic tobacco resistant to Tomato mosaic virus and Cucumber mosaic virus, using ToMV and CMV part of the CP fragment as interference target sequences,we constructed pBi35SG12RNAi expression vector. The RNAi vectors were transformed into wild-type Nicotiana occidentalis by Agrobacterium mediated transformation. The results showed that RNA interference sequence of the two viruses was introduced into the genorne of Nicotiana occidentalis successfully by PCR identification and kanamycin screening,and transgenic tobacco was confirmed to be resistant to ToMV and CMV significantly.

关 键 词：番茄花叶病毒 黄瓜花叶病毒 RNAI载体 转基因烟草 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S332.2[农业科学—植物保护]