口服VitaminE对离心运动后大鼠骨骼肌损伤的影响  

Influence of vitamin E on skeletal muscle injury of rats after eccentric exercise

作　　者：吴翊馨[1] 张海平[1] 高岩[1] 苏胜林[1] 

机构地区：[1]沈阳体育学院运动人体科学学院,110102

出　　处：《中国实用护理杂志》2013年第27期11-14,共4页Chinese Journal of Practical Nursing

基　　金：2012年国家社会科学基金项目“我国心脑血管疾病老年患者康复促进模式研究”（12CTY028）

摘　　要：目的从骨骼肌超微结构和肌浆网自由基代谢的角度，探讨维生素E（VitaminE）对离心运动后大鼠骨骼肌损伤的影响。方法取健康雄性SD大鼠48只，随机分为对照组、运动组、生理盐水组、VitaminE组，每组各12只，制作相应的动物模型。动物模型采用一次持续性下坡跑运动训练，运动结束后，取大鼠右侧肱三头肌，部分用于电镜标本制做，余下肌肉采用差速离心方法提取肌浆网，并进行丙二醛（MDA）含量和超氧化物歧化酶（SOD）活力测定。结果VitaminE组大鼠骨骼肌在离心运动后24h的损伤超微结构较生理盐水组改善明显，与运动组和生理盐水组比较，丙二醛显著降低，超氧化物歧化酶显著增高，但仍差于对照组。结论VitaminE可以降低骨骼肌肌浆网丙二醛含量、增加肌浆网超氧化物歧化酶＇活．陛，并使离心运动后骨骼肌损伤形态学改变得以改善。Objective From the angle of skeletal muscle uhrastructure and free radical metabolism, the paper explored the effect of vitamin E on skeletal muscle＇s damage after eccentric exercise. Methods 48 male Sprague Dawley rats were randomly divided into the control group, the sport group, the physiological saline group, and the vitamin E group. The model of animals was made by a bout of eccentric exercise on a treadmill. After the training,triceps brachiies were extracted from the right side of rats. Parts of triceps brachiies were made for microelectric slices, the others for the extracting of SR by the method of centrifugation at different speeds and for measuring the content of MDA and activity of SOD. Results In the vitamin E group, the muscular ultmstmctures damage were improved obviously compared with that of the physiological saline group. Meanwhile, the value of MDA in the vitamin E group was greatly decreased and activity of SOD was notably increased compared with the sport group and the physiological saline group, but still worse than those of the control group. Conclusions Supplying of vitamin E could decrease the content of MDA in SR of skeletal muscle, increase the activity of SOD, then fuaher relieve the effect of free radical on athletic injury.

关 键 词：VITAMIN E 离心运动 大鼠骨骼肌 丙二醛 超氧化物歧化酶 

分 类 号：R873[医药卫生—运动医学]