针刺对快速老化小鼠P8海马神经元突触可塑性及AMPA受体表达的影响  

The effect of acupuncture on AMPA receptor expression in the hippocampus of senescence-accelerated mice is correlated with synaptic plasticity

作　　者：封敏[1] 熊殷艺[2] 鲁娟[2] 张承舜[2] 于美玲[3] 吴巧凤[2] 卢圣锋[3] 余曙光[2] 

机构地区：[1]怀化医学高等专科学校针灸推拿系,怀化418000 [2]成都中医药大学针灸推拿学院 [3]南京中医药大学第二临床医学院

出　　处：《中华物理医学与康复杂志》2013年第9期677-681,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金（81072866,81202743）;湖南省高校科研项目（12C1202）;怀化医学高等专科学校科研项目（2012JY05）

摘　　要：目的研究针刺对快速老化小鼠P8（SAMP8）海马神经元突触可塑性及谷氨酸α-氨基-3-羟基-5-甲基4．异唑丙酸（AMPA）受体表达的影响。方法选取雄性9月龄SAMP819只，按随机数字表法分为模型组（10只）和针刺组（9只），另选取雄性同月龄抗快速老化小鼠（SAMR1）9只作为对照组。针刺组给予“百会”、“涌泉”穴位针刺治疗，每日1次，7d为1个疗程，疗程之间相隔2d，共治疗4个疗程。第4疗程内每次治疗后，采用Morris水迷宫实验评估动物的学习记忆能力。应用透射电镜观察治疗后小鼠海马CAl区神经元突触的超微结构变化，用Western blot法检测小鼠海马AMPA受体亚基谷氨酸受体1（GluR1）及谷氨酸受体2（GluR2）的含量。结果定位航行实验中，与对照组比较，模型组逃避潜伏期延长（P〈0．05），针刺组较模型组缩短（P〈0．05）。空间探索实验中，对照组、模型组、针刺组小鼠穿越有效区的次数分别为（5．33±2．29）次、（2．30±0．82）次、（5．22±2．05）次，与对照组比较，模型组穿越有效区的次数减少（P〈0．05），针刺组较模型组增加（P〈0．05）。与对照组比较，模型组小鼠海马CA1区神经元PSD[（39．83±9．30）nm]变薄（P〈0．05）、突触间隙[（19．98±5．21）nm]增宽（P〈0．05）、突触界面曲率（1．12-t-O．05）下降（P〈0．05）；与模型组比较，针刺组小鼠突触PSD[（46．78±11．37）nm]增厚（P〈0．05）、突触间隙[（15．68±4．03）nm]缩小（P〈0．05）、突触界面曲率（1．14±0．09）增大（P〈0．05）。模型组小鼠海马GluR1含量较对照组下降（P〉0．05），针刺组较模型组GluR1含量增加（P〉0．05），模型组GluR2含量较对照组下降（P〈0．05），针刺组较模型组GluR2含量增加（P〈0．05）。结论针刺可显著提高SAMP8的学习记忆能力及突触可塑性，并促进其海马AMPA受体GObjective To observe any effect of acupuncture on AMPA receptor expression and synaptic plasticity in the hippocampal neurons of mice prone to accelerated senescence （SAMP8s）. Methods Nineteen SAMP8s aged 9 months were randomly divided into a model group （n = 10） and an acupuncture group （n = 9）. Nine mice resistant to accelerated senescence （SAMR1 s） were used as a control group. Acupuncture was administered daily on the DU20 （Baihui） and K1 （Yongquan） acupoints, 7 days in each course for 4 courses, with a 2 day interval between courses. The Morris water maze was used to observe learning and memory abilities. The mice were sacrificed after the last treatment. Transmission electron microscopy was used to observe the synaptic ultrastrueture in subregion CA1 of the hippocampus. Western blotting was used to detect the expression of the AMPA receptor subunits GluR1 and GluR2. Results Compared to the control group, escaping latency was prolonged significantly in the model group but decreased in the acupuncture group. Target annulus crossings decreased significantly in the model group but increased significantly in the acupuncture group. Compared to the model group, the average thickness of the post-synaptic density （PSD） and the curvature of the synaptie interface both increased significantly in the acupuncture group, while the average synapse width decreased significantly. There were no significant differences in GluR1 expression among the groups, but compared to the control group, GluR2 expression decreased significantly in the model group but was significantly up-regulated in the acupuncture group. Conclusions Acupuncture can improve learning and memory, facilitate synaptic plasticity and up-regulate GIuR2 expression in hippocampal neurons, at least among SAMP8 rats.

关 键 词：针刺疗法 学习记忆 突触可塑性 AMPA受体 快速老化小鼠P8 

分 类 号：R245[医药卫生—针灸推拿学]