应用压电微生物传感仪研究五味消毒饮抑菌作用的量效关系  

Research on dose-effect relationship of Wuwei Xiaodu Drink on antibacterial metabolism by MSPQC

作　　者：张晓青[1] 陈伶利[1] 李斌[1] 黄莺[1] 许光明[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《中草药》2013年第18期2569-2572,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81202451);湖南省自然科学基金资助项目(12JJ4029);湖南省教育厅科研项目(11A083;11C0952;11C0943);湖南省高校科技创新团队资助项目(15);湖南省重点学科中西医结合基础资助(100601)

摘　　要：目的研究五味消毒饮对细菌生长、代谢的影响及量效关系。方法用多通道串联式压电传感仪(MSPQC)测定五味消毒饮对大肠杆菌、金黄色葡萄球菌、绿脓杆菌生长代谢影响的频移曲线,根据曲线中细菌的检出时间(FDT)以及频移的大小得出最低抑菌浓度(MIC)和最低杀菌浓度(MBC),绘制出五味消毒饮的质量浓度与FDT的线性关系曲线,进行量效关系分析。用传统试管稀释法进行平行实验。结果五味消毒饮对大肠杆菌、金黄色葡萄球菌、绿脓杆菌有不同程度的抑制作用,质量浓度越高抑菌效果越强,其中对大肠杆菌的抑制作用最强,24 h内对大肠杆菌、金黄色葡萄球菌、绿脓杆菌的MIC分别为0.7、0.8、0.8 g/mL,MBC均为0.9 g/mL。结论用MSPQC检测抑菌类中药的量效关系具有实时、灵敏、定量、快速等优势。Objective The effect of Wuwei Xiaodu Drink （WXD） on the metabolism of bacteria and the dose-effect relationship were investigated by MSPQC. Methods The frequency shift-time curves of the growth and metabolism of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa affected by WXD were obtained by using MSPQC. The minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） were gained according to FDT and the frequency shift. The dose-effect relationship was analyzed according to the curve which represented the relationship between the concentration of WXD and FDT. The parallel test was carried out with the traditional tube dilution method. Results The results indicated that WXD inhibited the growth of E. coli, S. aureus, and P. aeruginosa, which was dose-dependent. The higher the concentration was, the stronger the antibacterial effect became. The MBC of WXD for three kinds of pathogenic bacteria was 0.9 g/mL. The MIC of WXD for S. aureus and P. aeruginosa was 0.8 g/mL, however for E. coli was 0.7 g/mL within 24 h. Conclusion MSPQC is a sensitive, quantitative, quick method, which could provide the process information in real time for determining the dose-effect relationship of antimicrobial Chinese materia medica.

关 键 词：五味消毒饮 抑菌 最低抑菌浓度 最低杀菌浓度 量效关系 多通道串联式压电传感仪 

分 类 号：R285.5[医药卫生—中药学]