河南产不同种群茜草遗传多样性分析  

Analysis on genetic diversity of Rubia cordifolia from various geographical populations in Henan province

作　　者：董美芳[1] 袁王俊[2] 韩远记[1] 尚富德[1] 

机构地区：[1]河南大学生命科学学院,河南开封475004 [2]河南大学中药研究所,河南开封475004

出　　处：《中草药》2013年第18期2600-2604,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30970176);河南省高校青年骨干教师资助计划(2009GGJS-152)

摘　　要：目的 对河南7个野生种群的茜草遗传多样性进行调查，为茜草野生资源的保护提供科学依据。方法 通过DNA测序技术分析叶绿体基因片段psbA-trnH遗传变异。结果 psbA-trnH序列共得到40个单倍型（H1～H40），单倍型多样性指数为0.951 0±0.001 3，核酸多样性指数为0.014 77，遗传分化指数为0.081 53，种群间基因流为2.78。结论 河南地区野生茜草有很高的遗传多样性，种群间存在较强的基因流，种群间分化较小。Objective To investigate the genetic diversity of Rubia cordifolia from seven geographical populations in Henan province, and to provide evidence for the protection of R. cordifolia wild resources. Methods The autogenous variation of chloroplast gene fragment psbA-trnH was analyzed by DNA sequencing technique. Results Based on cpDNA data, 40 haplotypes （H1—H40） were identified, the diversity index of haplotype and nucleotide diversity index were 0.951 ± 0.001 3 and 0.014 77, respectively. Genetic differentiation index was 0.081 53, and gene flow among populations was 2.78. Conclusion The wild populations of R. cordifolia in Henan province have higher genetic diversity in geo-authentic product area than other species. There is less genetic differentiation among populations because of the stronger seed-mediated gene flow.

关 键 词：茜草 遗传多样性 单倍型 DNA测序 基因流 

分 类 号：R282.12[医药卫生—中药学]