大鼠肾缺血再灌注损伤程序性坏死的形态学观察  

Morphological observation of necroptosis after renal ischemia-reperfusion injury in rats

作　　者：李郭锦[1] 张路 穆长征[3] 宋小峰[3] 郭敏[3] 

机构地区：[1]辽宁医学院附属第三医院影像科,辽宁锦州121001 [2]盘锦市职业技术学院临床护理系,辽宁盘锦124010 [3]辽宁医学院组织胚胎学教研室,辽宁锦州121001

出　　处：《解放军医学杂志》2013年第10期792-795,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(31200871);辽宁省自然科学基金(201202141);辽宁医学院博士启动基金(20101311)~~

摘　　要：目的观察大鼠肾缺血再灌注损伤程序性坏死的形态学变化。方法应用免疫印迹技术、免疫组织化学染色技术以及光学和电子显微镜技术对缺血60min再灌注24h的大鼠肾脏进行观察和分析。结果免疫印迹分析结果表明,与假手术组比较,大鼠肾缺血再灌注后受体相互作用蛋白激酶3(RIPK3)和肿瘤坏死因子受体α(TNFRα)表达增强。缺血再灌注损伤的肾组织内出现RIPK3免疫组化染色阳性细胞,主要分布于肾小管上皮。光镜下皮质和髓质外带的肾小管出现了程序性坏死,表现为细胞肿胀,着色苍白,细胞核固缩。电镜下程序性坏死表现为细胞质肿胀,细胞器肿胀、破碎,含有一个凋亡样细胞核。结论大鼠肾脏缺血60min再灌注24h后部分肾小管细胞发生了程序性坏死,肾组织中RIPK3表达增强。Objective To observe the morphological changes of necroptosis after renal ischemia-reperfusion injury （RIRI） in rats. Methods Immunoblot analysis, immunohistochemical staining and light and electron microscope were used to observe and analyze necroptosis in the renal tissue after 60min ischemia and 24h reperfusion. Results Immunoblot analysis showed that the expression of receptor interacting protein kinase 3 （RIPK3） and tumor necrosis factor receptor α （TNFRα） in the kidney was significantly higher in RIRI group than in sham group. Immunohistochemical staining showed positive cells of RIPK3 in the kidney was evident in RIRI group, which distributed mainly in the renal tubular epithelium. Renal tubular cells undergoing necroptosis were evident in both cortical and outer region of the medulla after 60min ischemia and 24h reperfusion and morphologically featured by necrotic cytoplasm containing an apoptosis-like nucleus under electron microscope. Conclusion Necroptosis occurred in renal tubular cells and the expression of RIPK3 could increase in renal tubular cells in rat after 60min ischemia and 24h reperfusion.

关 键 词：肾 再灌注损伤 程序性坏死 大鼠 

分 类 号：R320.234.5[医药卫生—人体解剖和组织胚胎学]