毫米波辐照所致急性应激对小鼠细胞因子和Th1/Th2免疫平衡的影响  

Changes of mouse cytokines and Th1/Th2 balance in acute stress induced by millimeter-wave

作　　者：孙嵘[1] 马琼[1] 杜丽[1] 蔡金玲[1] 杨晓云[1] 高艳[1] 崔玉芳[1] 毛建平[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《解放军医学杂志》2013年第10期859-863,共5页Medical Journal of Chinese People's Liberation Army

基　　金：毫米波国家重点实验室开放课题(K200909;K201225);supported by Open Issues of State key Laboratory of Millimeter Waves (K200909; K201225)

摘　　要：目的探讨35GHz毫米波辐照诱发的小鼠急性应激细胞因子,尤其是Th1/Th2型因子的变化规律及其对免疫平衡的影响。方法 BALB/c小鼠经35GHz、420mW/cm2功率密度的毫米波源急性辐照60s,红外监测仪实时监测小鼠背部皮肤温度变化。于辐照后不同时间点检测小鼠外周血象、胸腺及脾脏指数,并用ELISA法检测血清中IL-1β、IL-2、IL-6及Th1/Th2型细胞因子IFN-γ、IL-4的变化。结果辐照开始后小鼠耳廓温度迅速升高到50℃,至辐照50~60s时升至约60℃。辐照后6h外周血白细胞(WBC)和淋巴细胞(LYM)出现明显的一过性降低,至辐照后1~7d基本恢复至对照水平。胸腺指数在辐照后6h短暂升高后逐渐降低,至辐照后7d明显低于对照组。脾脏指数在辐照后3、7d均出现明显升高。外周血IL-1β在辐照后6h即迅速升高至峰值,1d降至最低(P<0.05),3~7d逐渐恢复至对照水平;而IL-6在辐照后6h^3d明显升高,1d升至峰值,7d仍明显高于对照组(P<0.05)。Th1和Th2型细胞因子IFN-γ和IL-4在辐照后3d均升高至峰值,至7d仍明显高于对照组(P<0.01)。Th1/Th2(IFN-γ/IL-4)比值在辐照后1、3d分别增高为对照值的1.34和1.66倍(P<0.05)。结论 35GHz毫米波急性辐照小鼠能诱发明显的热效应,血清细胞因子应激性升高,Th1/Th2平衡明显向Th1免疫方向偏移,表明免疫调节在毫米波辐照诱发的急性应激反应中发挥了重要的调节作用。Objective To explore the regular pattern of variation of cytokines, especially the Th1/Th2 cytokines, and its effect on immune balance in stress induced by 35GHz millimeter-wave （MMW） exposure in mice. Methods BALB/c mice were exposed to 420mW/cm2, 35GHz MMW for 60s, and the dorsal skin temperature was real-time monitored with infrared monitor during the period of exposure. Peripheral WBC, thymic and splenic indexes of mice were detected at different time points after MMW exposure. Meanwhile, the contents of serum IL-1β, IL-2, IL-6 and Thl/Th2 cytokines IFN-γ, IL-4 were analyzed by ELISA. Results The temperature of ear auricle rapidly increased to 50℃ after initiation of exposure, and continuously rose to about 60℃ at 50s to 60s. Peripheral WBC and lymphocytes showed transient reduction at 6h and restored to the control level from 1 to 7d after radiation exposure. Compared with control group, thymus index displayed a transient increase at 6h and thereafter decreased, and then diminished to which significantly lower than control level 7d after radiation exposure. However, spleen index increased stgnificantly on day 3 and day 7 after radiation exposure. Peripheral IL-1β levels immediately increased to the maximum at 6h and decreased to the minimum at ld （P〈0.05）, then gradually recovered to control level during 3d and 7d after radiation exposure. However, IL-6 level displayed a significant increment from 6h to 3d, and increased to peak value at ld and still obviously higher than that of control group up to 7d （P〈0.05） after radiation exposure. Simultaneously, Th1/Th2 cytokines IFN-γ/and IL-4 both increased to the maximal at 3d and still significantly higher than those of control group at 7d （P〈0.01） after radiation exposure. Ratios of Th1/Th2 （IFN-γ/IL-4） evidently raised to 1.34 and 1.66 times of control values at ld and 3d respectively （P〈0.05）. Conclusions 35GHz MMW exposure can induce obvious thermal effects in mice, and serum cytokines increase due to stress, the Th1/T

关 键 词：毫米波 应激 细胞因子类 免疫平衡 

分 类 号：Q684[生物学—生物物理学]