一株H4N6亚型禽流感病毒A/duck/Guangdong/W8/2011(H4N6)的全基因组测序及遗传进化分析  

Sequencing and genetic evolution analysis of the entire genome of an A/duck/Guangdong/W8/2011(H4N6)avian influenza virus

作　　者：张长辉[1] 亓文宝[1] 田进[1] 李华楠[1] 黄丽红[1] 焦培荣[1] 徐成刚[1] 廖明[1] 

机构地区：[1]华南农业大学兽医学院农业部兽用疫苗创制重点实验室广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642

出　　处：《中国兽医科学》2013年第9期893-899,共7页Chinese Veterinary Science

基　　金：国家肉鸡产业技术体系项目(nycytx-42-G3-03)

摘　　要：为了解H4N6亚型禽流感病毒A/duck/Guangdong/W8/2011(H4N6)的来源、特征及分子演化规律,对该病毒进行了全基因组序列扩增,结合GenBank中已收录的H4N6亚型流感病毒及其他相关毒株序列进行了遗传进化分析。结果显示,该病毒的HA基因裂解位点附近的氨基酸序列为PEKASR↓GLF,符合典型低致病性禽流感病毒的特征。该病毒的HA、NA、PB2、PA和M基因与我国周边国家和地区的H4N6毒株亲缘关系较近,而其PB1、NP和NS基因与国内不同亚型的禽流感毒株亲缘关系较近;同时这些周边国家和地区均是候鸟迁徙的重要栖息地,这表明A/duck/Guangdong/W8/2011(H4N6)可能是由国外H4N6毒株随候鸟迁徙传入我国,并在鸭体内与其他禽流感毒株发生基因重组形成的重组病毒。To investigate the origin, characteristics and molecular evolution of duck-derived H4N6 sub- type avian influenza virus（AIV） isolate A/duck/Guangdong/W8/2011 （H4N6）, the entire genome of the isolate was amplified and subjected to genome sequencing. The Lasergene 7. 1 and MEGA 4. 0 software were used for sequence analysis and phylogenetic tree construction of A/duck/Guangdong/WS/2011 （H4N6） with some other reference sequences in GenBank. The results indicated that the amino acid motif of the HA gene cleavage site was PEKASR ~ GLF, which is the typical characterization of the low patho- genic AIV. The HA,NA,PB2,PA and M genes of the isolate had closely related to those of H4N6 viruses isolated from the countries and regions around China,where were the habitats of migratory birds^however, the PB1, NP and NS genes of the isolate had the greatly related to those of other subtype viruses isolated in China. The results showed that A/duck/Guangdong/WS/2011（H4N6） might be a novel reassortant among ducks,which genes may have originated from H4N6 viruses isolated from the countries around China and other viruses in China.

关 键 词：禽流感病毒 H4N6 分离鉴定 进化分析 

分 类 号：S852.659.5[农业科学—基础兽医学]