罗格列酮抗家兔动脉粥样硬化的实验研究  

Effects of rosiglitazone in rabbits with atherosclerosis

作　　者：慕仲元[1] 潘明[1] 白明[1] 李强[1] 李万玲[1] 

机构地区：[1]兰州大学第一医院心内科,兰州730000

出　　处：《临床心血管病杂志》2013年第9期661-663,共3页Journal of Clinical Cardiology

摘　　要：目的:观察罗格列酮对兔主动脉斑块基质金属蛋白酶(MMP)-2、基质金属蛋白酶抑制因子(TIMP)-2表达的影响。方法:健康纯系新西兰大白兔27只,随机分为对照组、模型组、罗格列酮组。实验10周末,酶法检测血脂水平,取主动脉弓标本,切片行苏木素-伊红(HE)染色,并采用免疫组织化学法检测兔主动脉粥样硬化斑块内MMP-2、TIMP-2的表达,并观察主动脉斑块形态学变化。结果:与模型组比较,罗格列酮组动脉粥样硬化斑块和泡沫细胞数减少,MMP-2表达显著降低,而TIMP-2表达显著增加(P<0.05)。结论:罗格列酮可通过下调兔主动脉粥样硬化组织中MMP-2及上调TIMP-2的表达,减轻大动脉内膜的炎症反应以及调节免疫等机制而发挥抗动脉粥样硬化作用。Objective：To observe the expression of matrix metalloproteinase （MMP）-2 and tissue inhibitors of metalloproteinase （TIMP） 2 in rabbits model with atherosclerosis. Method：The 27 healthy male pure New Zealand rabbits were randomly divided into control group, model group and rosiglitazone group. After l0 weeks of the experiment, enzymology method was used to detect tile levels of serum lipid. The expressions of the bistopatholo- gy and protein of MMP-2 and TIMP 2 in atheroselerotic plaques of the aorta were observed by immunohistochem- istry technique. Result： Compared with the model group, rabbit aorta atherosclerotic plaque and foam ceils decreased, MMP-2 decreased, TIMP 2 increased in rosiglitazone group （P〈0.05）. Conelusion：Rosiglitazone could downregulate MMP-2 and upregulate TIMP-2 in rabbits with atherosclerosis.

关 键 词：动脉粥样硬化 罗格列酮 基质金属蛋白酶-2 组织基质金属蛋白酶抑制因子-2 

分 类 号：R543.1[医药卫生—心血管疾病]