丝裂原活化蛋白激酶在白细胞介素8趋化心脏成纤维细胞中的作用  

Role of MAPKs in the migration procession of the fibroblasts induced by interleukin-8

作　　者：饶利亚[1] 赵欣[1] 刘宇宙[1] 石怀瑞[1] 李裕舒[1] 周聪[1] 言龙[1] 

机构地区：[1]华中科技大学协和医院心血管病研究所,武汉430022

出　　处：《临床心血管病杂志》2013年第9期695-698,共4页Journal of Clinical Cardiology

基　　金：武汉市科技局重点资助项目(No:201060938361)

摘　　要：目的：研究白细胞介素8（IL-8）是否能够诱导心脏成纤维细胞（CF）的迁移运动,并验证该过程是否涉及丝裂原活化蛋白激酶（MAPK）亚家族ERK1/2、P38、c-JNK信号转导通路。方法：应用Transwell技术研究IL-8对CF的趋化诱导作用,以及MAPK信号转导通路与这种迁移的关系。结果：①IL-8在10-8~10-10 g/ml时均能诱导CF迁移运动,并在10-9 g/ml时迁移效应最明显;②IL-8诱导CF的迁移作用开始于4~8h,并呈现时间依赖性;③MAPK亚家族ERK1/2、P38、c-JNK信号转导通路在IL-8诱导的CF迁移过程中均发挥作用。结论：IL-8能够诱导CF迁移运动,且这种迁移过程可能有MAPK亚家族ERK1/2、P38、c-JNK信号转导通路参与。Objective：To test weather interleukin-8 （IL-8） could induce the cardiac fibroblasts （CF） migration and mitogen-activated protein kinase （MAPK） signaling pathways including ERK1/2, P38, c-JNK were involved in the migration process. Method= The migration and effect of the MAPK signaling pathways involved were esti- mated by transwell chamber in vitro. Result= （I）IL-8 stimulated the migration function of CF at the concentration range of 10 8 g/ml to 10 10 g/ml, especially at 10 ^-9g/ml. （2）The migration began at 4--8 h with a dose- dependent manner. （3）All three MAPK pathways including ERK1/2, P38, c-JNK took effect on the migration process. Conclusion：IL-8 could induce CF migration via all three MAPK signaling pathways including ERK1/2, P38, and c-JNK.

关 键 词：心脏成纤维细胞 白细胞介素8 迁移 MAPK 

分 类 号：R542.2[医药卫生—心血管疾病]