E16.5小鼠胚胎胰腺组织移植治疗实验性糖尿病  

E16.5 embryonic mouse pancreatic tissue transplantation for the treatment of experimental diabetes

作　　者：杨军[1] 周光文[2] 

机构地区：[1]南通大学附属无锡市第三人民医院外科,江苏省214041 [2]上海交通大学医学院附属第一人民医院外科

出　　处：《中华普通外科杂志》2013年第9期705-708,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（编号：81170721）

摘　　要：目的 以小鼠E16.5胚胎胰腺组织移植为例,探索早期胚胎胰腺组织移植对糖尿病的治疗效果.方法 STZ诱导建立雄性C57BL/6小鼠糖尿病模型,随机分成移植组（TX组）、糖尿病假手术组（SO组）.TX组（n=9）：C57 BL/6小鼠肾被膜下移植5～7个E16.5 d胚胎胰腺组织,SO组（n=6）：肾被膜下注入50μl RPMI1640培养液.每日记录小鼠的体重,每周两次检测小鼠血糖水平.TX组小鼠血糖水平≤11.2 mmol/L后,IPGTT和IPITT方法观察移植的胚胎胰腺组织内分泌功能,摘除移植物后观察小鼠的血糖变化.ELISA方法检测胚胎血清、移植前后E16.5胚胎胰腺组织（3.3 mmol/L和16.7 mmol/L）糖刺激胰岛素分泌量.观察移植前后E16.5胚胎胰腺组织胰岛素和胰高血糖素表达情况.结果 TX组移植后4～6周,受体血糖较移植前降低（13.4 ±6.5比28.9±2.5,P＜0.05）,体重增加（P＜0.05）；与SO组相比较,TX组高血糖症状明显得到控制（P＜0.001）.IPGTT和IPITT结果表明移植的胚胎胰腺组织可根据血糖水平变化调节胰岛素分泌.ELISA结果示E16.5小鼠胚胎组织已有胰岛素分泌,移植后受体的胚胎胰腺组织较移植前胰岛素分泌量显著增加.免疫组织化学示移植后E16.5胚胎胰腺组织胰岛素和胰高血糖素的表达水平显著高于移植前.结论 早期胚胎胰腺组织移植能有效表达分泌胰岛素,调控受体血糖,使受体的体重和血糖达到正常水平.Objective To investigate the effect of early embryonic mouse pancreatic tissue transplantation in the treatment of mouse experimental diabetes. Methods C57BL/6 mouse models of streptozocin-induced diabetes mellitus were established and then randomly divided into two groups： transplantation group, in which, five to seven pieces of pancreatic tissue of mice at embryonic 16. 5 days were transplanted into mouse renal capsule, and sham-operated （ SO ） control group, in which, 0. 05 ml RPMI1640 culture medium was injected into mouse renal capsule. When blood glucose level of the transplantation group mouse was ~〈 11.2 mmol/L, the endocrine function of embryonic pancreatic tissue transplanted was detected by IPGTF and IPITY methods and then the transplanted graft was removed for observing the blood glucose surge. In vitro, levels of insulin secretion were measured in serum, E16. 5 pancreatic tissue and graft （3.3 mmol/L and 16. 7 mmol/L） glucose stimulation by ELISA. Histology and immunohistoehemistry were observed before or after pancreatic tissue transplantation to detect insulin and glycagon production. Results （ 1 ） The glucose level decreased significantly in the TX group after 4 - 6 weeks of transplantation （ 13.4 _＋ 6. 5 vs 28.9 ＋ 2. 5, P 〈 0.05 ） , and the body weight gaining （ P 〈 0. 05 ）. Compared to SO group, glucose level decreased significantly （ P 〈 0. 001 ）. The graft can modulate insulin secretion; （2） Insulin can be detected in El6. 5 embryonic mouse. Insulin and glycagon were produced more in TX group than pretransplantated pancreatic tissue. Conclusions El6. 5 embryonic mouse pancreatic tissue transplantation effectively controls serum glucose level and restore the nondiabetic pattern of weight gaining in diabetic mouse.

关 键 词：糖尿病 胚胎结构 移植 小鼠 

分 类 号：R587.1[医药卫生—内分泌]