GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化  

Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats

作　　者：同海妮[1,2] 白淑[1,2] 王树迎[1,2] 黄丽波[1,2] 石运芝[1,2] 侯衍猛[1,2] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省动物生物技术与疫病防治重点实验室,泰安271018

出　　处：《畜牧兽医学报》2013年第9期1392-1399,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30871832);山东省自然科学基金(ZR2009DM027);山东省科技攻关项目(2008GG30008018)

摘　　要：旨在探讨济宁青山羊生后发育过程中生长激素受体(Growth hormone,GHR)和胰岛素样生长因子I受体(Insulin-like growth factor-I,IGF-IR)在子宫的分布及其mRNA的表达规律。运用实时荧光定量PCR技术,检测GHR mRNA和IGF-IR mRNA的表达规律;SABC法检测GHR和IGF-IR免疫阳性细胞在子宫的分布。结果表明,GHR和IGF-IR免疫阳性染色主要见于子宫腺管上皮、基质、血管内皮和子宫平滑肌细胞的胞质中,偶见胞核着色。GHR和IGF-IR的阳性细胞数与其mRNA表达的变化趋势基本一致,且IGF-IR低于GHR。GHR和IGFIR在初情期(60日龄)和性成熟(120日龄)达到高峰(P<0.01),120日龄后下降,并维持在一个相对稳定的水平。GHR和IGF-IR在子宫的生长发育过程中起着重要作用,且初情期和性成熟期可能是济宁青山羊生后子宫发育最快的时期。To study the localization of GHR and IGF-IR and the expression of GHR mRNA and IGF-IR mRNA in uterus of Jining Grey goats during postnatal development. Real-time floures- cence quantitative PCR was used to detect the expression pattern of GHR mRNA and IGF-IR mRNA, and SABC method was applied to research the distribution of positive cells of GHR and IGF-IR in the uterus. GHR and IGF-IR were mainly localized in the cytoplasm of glandular epi- thelium, stromal cells, endothelium and smooth muscle cells of the uterus, occasionally in the nuclei. The changed trends on the positive cells of GHR and IGF-IR were similar with that on the expression of its mRNA. Although the expression of IGF-IR was less than that of GHR, the ex- pression of GHR and IGF-IR both reached its peak on the puberty （D60） and the sexual maturity （D120）（P〈0.01）, Then decreased and remained at a relatively stable level. GHR and IGF-IR play an important role in the growth of the uterus, and the puberty and the sexual maturity may be the fastest period of the uterus during postnatal development.

关 键 词：济宁青山羊 子宫 GHR IGF-IR 免疫组化法 QRT-PCR 

分 类 号：S827[农业科学—畜牧学] S811.2[农业科学—畜牧兽医]