青藏高原细粒棘球绦虫的分子鉴定与遗传变异分析  

Molecular Identification and Genetic Variation of Echinococcus granulosus in the Tibetan Plateau

作　　者：胡丹丹[1] 王凝[1] 钟秀琴[1] 王家海[1] 延宁[1] 阳爱国 蒋忠荣[3] 郭莉 邓世金 达瓦次仁 孔维淑 刘天宇[1] 周旋[1] 谢跃[1] 古小彬[1] 杨光友[1] 

机构地区：[1]四川农业大学动物医学院,雅安625014 [2]四川省动物疫病控制中心,成都610041 [3]四川省甘孜州畜牧科学研究所,康定626000 [4]西藏自治区动物疫病预防控制中心,拉萨850000 [5]西藏那曲地区职业技术学校,那曲852000

出　　处：《畜牧兽医学报》2013年第9期1438-1444,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支持计划项目(2006BAI06B09);国家质检总局科技计划项目(2009IK019)

摘　　要：为了探索青藏高原细粒棘球绦虫的遗传变异特点与系统发生关系,为该地区细粒棘球蚴病的分子诊断、流行病学和防控研究提供基础资料。本研究对采自青藏高原的43株细粒棘球蚴的线粒体ND5基因全序列进行测序及分析,共鉴定出42个分离株属于细粒棘球绦虫G1型,1个西藏绵羊分离株属于细粒棘球绦虫G6型。G1型分离株中,共有43个变异位点,分为27个单倍型,单倍型多样性为0.940±0.028,核苷酸多样性为0.001 93±0.001 14,单倍型之间的平均遗传距离为0.002 6。单倍型网络图以H9为优势单倍型,其余单倍型围绕它呈辐射状。单倍型歧点分布分析呈单峰,中性检验得到显著的负值。结果表明青藏高原细粒棘球绦虫流行的主要基因型为G1型,且种内变异较小。The aim of current study was to identify the genotype and genetic variation of Echino- coccus granulosus in the Tibetan plateau of China, and provide the basic molecular data needed for studies of the molecular diagnosis, epidemiology, prevention and control of echinococcus disea- ses. The whole mitochondrial ND5 gene sequences of 43 isolates from the Tibetan plateau were analyzed. We found out that 42 isolates were belonged to E. granulosus G1 genotype, while one isolate from Tibetan sheep was identified as E. granulosus G6 genotype according to phylogenetic tree. There were 43 mutation sites in G1 isolates, which were separated in 27 haplotypes. Thehaplotype diversity and nuclear diversity were 0. 940±0. 028 and 0. 001 93±0. 001 14, respective- ly; And mean genetic distance between haplotypes was 0. 002 6. Parsimony network showed a ra- dialized expansion from a main founder haplotype Hg. The analysis of mismatch distribution showed a unimodal structure. Additionally, significant negative neutrality indices were detected. All these results suggested that the main genotype of E. granulosus in the Tibetan plateau was G1 with low genetic variability.

关 键 词：细粒棘球绦虫 线粒体ND5基因 青藏高原 分子鉴定 遗传变异 

分 类 号：S852.734[农业科学—基础兽医学]