ELISA与IFA用于检测J亚型禽白血病病毒的比较分析  

Comparison of ELISA and IFA in the Detection of the J Subgroup Avian Leukosis Virus

作　　者：王鑫[1] 崔治中[2] 龚建森[1] 高明燕[1] 范建华[1] 俞燕[1] 朱静[1] 徐步[1] 

机构地区：[1]江苏省家禽科学研究所,扬州225125 [2]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2013年第9期1499-1503,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：公益性行业(农业)科研专项(#201203055)

摘　　要：为比较ELISA与IFA用于检测J亚型禽白血病病毒上的差别，将5个不同梯度TCID50的NX0101病毒接种到DF-1细胞上，维持培养6个不同时间段（1、2、3、5、7和9d），比较2种方法检测结果的相关性。试验发现接种剂量高于10^1.625 个TCID50，维持培养2～3dIFA即可检出阳性结果，而ELISA检测P27抗原相对滞后2～4d；当接种剂量低于10^0.625个TCID 50 时，维持培养至7dIFA检测结果为阳性而ELISA检测仍为阴性。由此得出结论IFA能快速、准确地检测到病毒的感染，而ELISA检出时间有明显滞后性。For comparing ELISA and IFA in the detection of J subgroup avian leukosis virus, five different dilution of NX0101 TCID50 virus were inoculated to DF1 cell and cultured. In six differ- ent stages （1, 2, 3, 5, 7 and 9 days culturation）, ELISA was applied to detect P27 antigen with- in supernatant and IFA was conducted to detect the infected cells respectively. Using more than 10^1.625 TCID50 of the inoculation dose, IFA achieves identification of all the infected cells at 2 days and 3 days post inoculation, but the results from ELISA is relatively lag of 2-4 days for confirma- tion. Inoculating less than 10^1.6.25 TCID50 of virus, IFA detect successfully the infected cells while ELISA still not detect by 7th day. The results suggest that IFA is more accurate and effective than commercial ELISA kit, ELISA have obvious hysteresis in comparison to IFA.

关 键 词：J亚群禽白血病病毒 酶联免疫吸附试验 间接免疫荧光试验 

分 类 号：S854.43[农业科学—临床兽医学]