1株野鸟源H5N2亚型禽流感病毒对SPF鸭的致病性分析  

Pathogenicity of SPF Ducks Infected with the Low-pathogenic Avian Influenza Virus H5N2 Subtype Isolated from Wild Birds

作　　者：管雪婷[1,2] 刘景利[3] 柴洪亮[1] 程成[1] 张翼[3] 左欣悦[3] 陈晓妹[3] 刘熠楠[1] 华育平[1] 李雁冰[3] 

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040 [2]东北农业大学动物科学技术学院,哈尔滨150030 [3]中国农业科学院哈尔滨兽医研究所,哈尔滨150001

出　　处：《畜牧兽医学报》2013年第9期1504-1508,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家林业局野生动物疫源疫病监测项目(2009)

摘　　要：旨在初步评估野鸟源H5N2亚型禽流感病毒（AIV）对幼龄鸭的致病力，为研究AIV的致病机制和禽流感发生机制提供理论依据。本研究以2周龄SPF鸭（绍兴麻鸭）为模型，模仿自然感染1株H5N2亚型LPAIV，对其致病性进行了初步研究。结果表明，试验鸭临床症状不明显，病毒在鸭消化道更能进行有效复制，排毒时间较长，具有直接接触感染的能力。病毒感染鸭7～14d可产生HI抗体，在第3周达最高值。病毒仅能在盲肠扁桃体和法氏囊2个器官组织中较高水平复制，却对法氏囊、肺、胸腺和胰腺造成不同程度的损伤。结果提示，幼龄鸭在AIV传播中起到一定作用，至于是否与真正自然状态下的感染有差异，还需进一步试验证实。This experiment was conducted to study the pathogenicity of the H5N2 avian influenza virus isolates from wild bird and investigate its pathogenic and occurring mechanism. Two-week- old SPF duck （Shaoxing sheldrake） was infected by an isolate of LPAIV HSN2 subtype as natural infection model in this study. The results showed that virus can propagate more effectively in di- gestive tract. Despite of absent of obvious clinical symptoms, the infected SPF duck shed virus for a longer time. The virus has the infection ability by direct contact. The antibody against HI can be detected at 7-14 days after infection, and the antibody level reached its peak at the third week after infection. Besides, this virus can propagate with higher level only in caecum tonsil and bursa of fabricius, while the tissue damage occurred in bursa of fabricius, lung, thymus and pan- creas in varying degrees. The results indicated that duckling plays a key role during AIV spread, whether it （even wild duck） cause by certain differences between the laboratory imitation natureand true nature infection, it remains to be further explored.

关 键 词：致病性 低致病性禽流感病毒 H5N2 SPF鸭 野鸟 

分 类 号：S852.659.5[农业科学—基础兽医学]