山楂酸对顺铂所致大鼠肾毒性的保护作用  

Protective effect of maslinic acid on nephrotoxicity induced by cisplatin in rats

作　　者：欧阳资章[1,2] 朱少华[3] 邬淑红 常惠礼[1,2] 陈方[1,2] 刘晓萍[1,2] 麦露丝[1,2] 肖诚胤[1,2] 

机构地区：[1]暨南大学附属清远医院 [2]清远市人民医院,广东清远511515 [3]中山大学药学院,广东广州510006 [4]清远市妇幼保健院,广东清远511500

出　　处：《中国新药与临床杂志》2013年第9期727-730,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：清远市科技局课题项目(2012B011204020)

摘　　要：目的探讨山楂酸对顺铂急性肾毒性的保护作用及作用机制。方法 40只SD雄性大鼠随机分为对照组(予等剂量生理盐水)、顺铂组(顺铂5 mg·kg^(-1))、山楂酸组(山楂酸40 mg·kg^(-1))、顺铂(5 mg·kg^(-1))+低剂量山楂酸(10 mg·kg^(-1))组、顺铂(5 mg·kg^(-1))+高剂量山楂酸(40 mg·kg^(-1))组,每组8只。顺铂给药方式为腹腔注射,山楂酸给药方式为经口灌胃,干预组腹腔注射顺铂前1 h经口灌胃给予山楂酸。均每日一次,连续两周。给药两周后,检测尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)、血清肌酐(Scr)和尿素氮(BUN)、肾组织丙二醛(MDA)和谷胱甘肽(GSH)含量。结果与对照组相比,顺铂组大鼠血清BUN和Scr、尿NAG酶、肾脏组织MDA含量显著升高,肾组织GSH含量显著下降(均P<0.01)。采用山楂酸干预后,大鼠血清BUN和Scr、尿NAG酶、肾脏组织MDA含量较顺铂组显著下降,GSH含量明显上升(P<0.05或P<0.01)。结论山楂酸对顺铂所致大鼠肾毒性有保护作用,其机制可能与抗氧化有关。AIM To evaluate the protective cisplatin and its mechanism. METHODS Forty male （8 in each） ： control group （same volume of normal acid group （maslinic acid 40 mg·kg-1） , cisplatin （5 role of maslinic acid against nephrotoxicity induced by Sprague-Dawley rats were randomly divided into 5 groups saline）, cisplatin group （cisplatin 5 mg· kg-1） , maslinic mg·kg-1） ＋ low-dose maslinic acid （10 mg·kg-1） groupand cisplatin （5 mg·kg-1） ＋ high- dose maslinic acid （40 mg ·kg-1） group. The cisplatin was given by intraperitoneal injection and maslinic acid by intragastric administration. The rats in each group were treated once a day for two weeks. The levels of urinary N-acetyl-β-D-glucosaminidase （NAG）, serum creatinine （Scr） and blood urea nitrogen （BUN）, malondialdehyde （MDA） and glutathione （GSH） in the kidney tissue were measured respectively. RESULTS The levels of BUN, Scr, urinary NAG, kidney tissue MDA concentration increased in the cisplatin compared with those in the control group and maslinic acid group （P 〈 0.01 ）, while the level of GSH in kidney tissue decreased （P 〈 0.01 ）. Maslinic acid could significantly reduce levels of BUN, Scr, urinary NAG and kidney tissue MDA concentration and increase the level of GSH in kidney tissue （P 〈 0.05 or P 〈 0.01 ）. CONCLUSION Maslinic acid might protecte the renal tissue from nephrotoxicity induced by cisplatin through anti-oxidation.

关 键 词：山楂酸 顺铂 药物毒性 肾毒性 大鼠 

分 类 号：R286[医药卫生—中药学]