FISH技术在芸薹属作物基因组研究中的应用进展  

Recent Advances in Brassica Genome Research by Fluorescence in situ Hybridization(FISH)

作　　者：轩淑欣[1] 冯大领[2] 李岩宾[1] 赵玉靖[1] 张成合[1] 申书兴[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071000 [2]河北农业大学生命科学学院,河北保定071000

出　　处：《园艺学报》2013年第9期1710-1718,共9页Acta Horticulturae Sinica

基　　金：国家‘863’课题(2012AA100202);国家自然科学基金项目(31171964);国家教育部高校博士点基金项目(20101302110001);河北省青年科学基金项目(C2010000738)

摘　　要：FISH技术是进行基因和重复DNA序列在染色体上可视化作图的精确有效方法,已广泛应用于特异核苷酸序列的物理作图、基因组或染色体的识别、DNA序列的定量分析以及着丝粒、染色质空间结构分析等方面。综述了FISH技术在芸薹属作物基因组研究中的重要进展,并讨论了其应用前景。Fluorescence in situ hybridization （FISH） is an effective and accurate method to visualize the localization of genes and repetitive DNA sequences on chromosomes. This technique has been widely used for physical mapping of unique nucleotide sequences on specific chromosome regions, for identifying the specific genomes and chromosomes, for mearuring the size of various DNA molecules, and for revealing the spatial organization of the centromere and heterochromatin or euchromatin of different chromosomes. The present review mainly described the recent advances in Brassica genome research by fluorescence in situ hybridization and discussed its future applications in Brassica crops.

关 键 词：芸薹属作物 荧光原位杂交 细胞遗传学 染色体涂染 伸展DNA纤维 

分 类 号：S63[农业科学—蔬菜学]