转基因育种技术在菊花性状改良中的应用进展  

Advances in Application of Transgenic Breeding Technology in the Traits Improvement of Chrysanthemum

作　　者：成丽娜[1] 魏倩[1] Muhammad Imtiaz 高俊平[1] 洪波[1] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100193

出　　处：《园艺学报》2013年第9期1813-1825,共13页Acta Horticulturae Sinica

基　　金：北京市园林绿化局计划项目(YLHH201300104)

摘　　要：自1989年发现了菊花对农杆菌浸染的敏感性,农杆菌介导转化法已成功应用于菊花花色、花期、株形、抗病虫及耐非生物胁迫等性状的改良中。从影响菊花遗传转化效率的农杆菌菌株类型、外植体选用、辅助添加剂的使用以及抗生素筛选等几方面阐述了菊花遗传转化技术研究的进展和应用现状,对菊花分子育种性状改良和重要功能基因发掘等研究进展进行综述,并对菊花转化效率改善途径的探索和环境友好型转基因新技术研发前景提出展望,以期为菊花分子育种提供参考。Following the discovery of chrysanthemum sensitivity to Agrobacterium in 1989, the technique of Agrobacterium-mediated genetic transformation has been successfully used in improvement of traits such as flower color, plant style, flowering time, disease-insect resistance and abiotic stress tolerance. In this review, the advances of Agrobacterium-mediated genetic transformation in chrysanthemum were summarized, including Agrobacterium strains, explants for infection, the addition of chemical compounds and selection markers. Also, the improvement of traits, exploration of important genes was reviewed and the perspective of enhancing transformation efficiency and developing environment-friendly transgenic technology were discussed. The main goal is to provide reference for molecular breeding of chrysanthemum.

关 键 词：菊花 农杆菌 转基因 分子育种 性状改良 

分 类 号：S682.11[农业科学—观赏园艺]