氟芬那酸丁酯软膏对SKH－1无毛小鼠的光保护作用  

Photoprotectlve effect of butyl flufenamate ointment on SKH-1 hairless mice

作　　者：吕婷[1] 涂庆峰 王秀丽 王宏伟[1] 

机构地区：[1]复旦大学附属华东医院皮肤科,上海200040 [2]上海市皮肤病医院

出　　处：《中华皮肤科杂志》2013年第10期711-715,共5页Chinese Journal of Dermatology

基　　金：上海市自然科学基金（11ZR1432800）;上海市级医院适宜技术联合开发推广应用项目（SHDC12010211）：上海市卫生局A类重点项目（20124034）

摘　　要：目的探讨氟芬那酸丁酯软膏能否对紫外线（uv）致SKH-1无毛小鼠日晒伤、皮肤光老化及皮肤鳞状细胞癌提供光保护作用。方法128只SKH-1无毛小鼠随机分为uV组、氟芬那酸组（氟芬那酸丁酯软膏＋uv照射）、基质组（基质＋uv照射）和空白组。以1．5倍最小红斑量的uV单次照射建立急性日晒伤模型（n=24），24h后观察皮肤红肿情况，免疫组化检测组织中COX-2表达；以90％最小红斑量为初始剂量，每周照射4次，连续12周和28周，分别建立光老化模型（n=24）和皮肤鳞癌模型（n=80）。12周后Masson染色观察小鼠光老化模型的胶原改变，免疫组化检测组织中Bax、Bcl-2和Caspase3水平。12～28周，记录小鼠鳞状细胞癌模型出现的肿瘤。结果氟芬那酸丁酯软膏预处理抑制uV照射引起的急性红肿反应（P〈0．05），降低COX-2的表达（P〈0．05）。12周后，氟芬那酸丁酯软膏减轻皮肤老化，Masson染色显示，该组真皮层胶原带密度高于其他uv组（P〈0．05）。同时免疫组化显示，氟芬那酸组较其他uV组下调Bcl-2，上调Bax和Caspase3的表达（P〈0．05）。连续28周，氟芬那酸丁酯软膏对小鼠无瘤生存期的影响与其他uV组相比差异有统计学意义（均P〈0．05），推迟了肿瘤的出现。结论氟芬那酸丁酯软膏具有-定的光保护作用。Objective To investigate the protective effect of butyl flufenamate ointment against ultraviolet （UV）-induced skin damage, skin aging, and cutaneous squamous cell carcinoma （CSCC） in SKH-1 hairless mice. Methods A total of 128 mice were randomly and equally divided into four groups： UV group receiving UV irradiation only, butyl flufenamate ointment group and matrix cream group receiving UV irradiation after 30-minute pretreatment with topical butyl flufenamate ointment and matrix cream respectively, and blank control group receiving neither pretreatment nor irradiation. In the sunburn experiment （n = 24）, mice were exposed to single session of UV irradiation （1.5 minimal erythema doses （MEDs））, and 24 hours later, erythema and swelling response was observed, and skin tissue was obtained from the irradiated area on the back of mice followed by the determination of COX-2 expression using the streptavidin biotin peroxidase complex （SABC） method. To establish a photoaging （n = 24） and CSCC （n = 80） model, mice were exposed to four sessions of UV irradiation every week for 12 and 28 successive weeks respectively, with the irradiation dose starting at 0.9 MED and increasing gradually. After 12-week irradiation, skin tissue was resected from the back of photoaged mice and subjected to Masson staining for the evaluation of collagen changes as well as immunohistochemical analysis for the quantification of Bax, Bcl-2 and Caspase 3 expression. The initiation and progression of CSCC were observed in mice on a once-a- week basis from 12 to 28 weeks. SPSS 21.0 software was used for statistical analysis. One way analysis of variance was carried out for multiple-group comparisons of numerical data, Ridit analysis for the comparison of immunohistochemical staining intensity. Kaplan-Meier method and log-rank test were utilized for the comparison of tumor-free survival time. Results Both the degree of erythema and swelling response and expression level of COX- 2 were significantly lower in the buty

关 键 词：氟芬那酸丁酯软膏 紫外线 光 晒伤 小鼠 

分 类 号：R965[医药卫生—药理学]