集胞藻Slr1515基因提高转基因水稻植株剑叶长度及叶绿素含量  

Increased Chlorophyll Content and Flag Leaf Length in Transgenic Rice Expressing Slr1515 of Synechocystis sp. PCC 6803

作　　者：杨浩萌[1] 薛哲勇[1] 赵乐[1] 漆小泉[1] 黄芳[1] 

机构地区：[1]中国科学院植物研究所,北京100093

出　　处：《植物学报》2013年第5期489-497,共9页Chinese Bulletin of Botany

基　　金：转基因生物新品种培育重大专项(No.2009ZX08009-121B)

摘　　要：蓝藻是古老的原核光合生物,不仅在植物进化上有重要地位,也是可利用的生物资源及重要的基因资源。Slr1515是来源于集胞藻6803(Synechocystis sp.PCC 6803)的基因,其同源基因在蓝藻中普遍存在,但对其生物学功能所知有限。以易于转化的水稻品种中花11(Oryza sativa subsp.japonica cv.‘Zhonghua 11’)为材料,通过农杆菌介导的遗传转化方法将Slr1515基因导入水稻胚性愈伤组织中。经过诱导分化,获得了一系列转基因幼苗。通过PCR、GFP荧光及免疫印迹检测,筛选出单拷贝插入的转基因阳性株系,并对野生型和转基因株系的表型进行比较分析。结果表明,灌浆期转基因株系的剑叶长度比同期的野生型提高了27.7%。进一步的测定结果显示,转基因株系的剑叶叶绿素含量和PSII最大量子产率分别比野生型提高了0.65倍和4.4%。推测Slr1515基因可能在改善水稻农艺性状方面有一定价值。Cyanobacteria are unique prokaryotes performing plant-like photosynthesis and important organisms with valuable genes. We cloned Sir1515 from the cyanobacterium Synechocystis sp. PCC 6803. The Sir1515 was introduced into rice （Oryza sativa L. subsp, japonica cv. ＇Zhonghua 11＇） by Agrobacterium-mediated transformation and verified by PCR and immunoblot analysis. Transgenic rice seeds were selected by GFP reporter signals, then seedlings underwent phenotype analysis. Agronomic traits such as chlorophyll content and flag leaf length were better in transgenic plants carrying Sir1515 than non-transgenic plants. These observations provide preliminary information for improvement of rice performance.

关 键 词：叶绿素含量 剑叶长度 水稻 Slr1515 蓝藻 

分 类 号：S511[农业科学—作物学]