大豆转录因子GmERF5的克隆、表达及功能分析  

Cloning, Expressing and Functional Analysis of GmERF5 from Soybean

作　　者：翟莹[1] 杨晓杰[1] 孙天国[1] 赵艳[1] 余春粉 王秀文[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,遗传重点实验室,齐齐哈尔161006 [2]长春市农业学校,长春130504

出　　处：《植物学报》2013年第5期498-506,共9页Chinese Bulletin of Botany

基　　金：齐齐哈尔大学青年教师科研启动支持计划(No.2012k-M20)

摘　　要：ERF转录因子是植物中特有的转录因子家族之一,在植物响应生物和非生物胁迫过程中发挥重要的调控作用。通过对大豆(Glycine max)吉林32未成熟胚的表达谱分析,利用RT-PCR技术从大豆中克隆了1个新的ERF转录因子GmERF5。GmERF5具有237个氨基酸残基,分子量为26.09 kDa,等电点为6.85,其开放阅读框长714 bp。该转录因子蛋白与GhERF2蛋白的同源性最高,它们同属ERF亚家族的第IV亚类。实时荧光定量PCR分析表明,该蛋白基因在大豆的根中表达量最高,且受干旱、高盐、低温及乙烯、脱落酸和茉莉酸甲酯的诱导上调表达。亚细胞定位实验结果表明,GmERF5蛋白定位于细胞核中。转录激活能力分析结果显示,GmERF5可以激活报告基因的表达,为转录激活子。综合以上结果,认为GmERF5可能作为转录调控因子参与大豆生物和非生物胁迫的应答。Ethylene-responsive factors （ERFs） are a subfamily of the AP2/ERF transcription factor superfamily in plant, whose members play an important role in regulating gene expression in plant responses to biotic and abiotic stresses. A soybean mRNA sequence encoding an ERF protein was cloned and designated GmERF5 from gene expression profiles of Jilin 32 immature embryo by use of RT-PCR. The GmERF5 consists of an ORF of 714 bp and encodes 237 amino acids （26.09 kDa） with an isoelectric point of 6.85. GmERF5 is grouped with Class IV ERF transcription factors and is related most closely to GhERF2. Real-time quantitative RT-PCR analysis revealed that GmERF5 expressed highly in root. GmERF5 was upregulated when Jilin 32 was treated by drought, high salt, low temperature, ethylene, abscisic acid and methyl jasmonate. GmERF5 protein was localized in the nucleus by subcellular localization assay in onion epidermal cells. The regulation ability of GmERF5 was analyzed in tobacco leaves by transient expression. GmERF5 activation of a reporter gene indicated that GmERF5 had transcription activation activity. GmERF5 may be involved in soybean biotic and abiotic stress responses as a transcription factor.

关 键 词：ERF转录因子 表达分析 大豆 亚细胞定位 

分 类 号：S565.1[农业科学—作物学]