基于药代动力学参数分析比较葛根芩连汤的不同配伍肠吸收特性  被引量:9

Analysis and comparison of intestinal absorption of components of Gegenqinlian decoction in different combinations based on pharmacokinetic parameters

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作  者:张艺竹[1] 安叡[1] 袁瑾[1] 王跃[1] 顾青青[1] 王新宏[1] 

机构地区:[1]上海中医药大学,上海201203

出  处:《药学学报》2013年第10期1611-1617,共7页Acta Pharmaceutica Sinica

基  金:2012年上海市自然科学基金面上项目(12ZR1431500);上海市教育委员会科研创新面上项目(11YZ69)

摘  要:基于药代动力学参数分析比较葛根芩连汤不同配伍组中葛根素、黄芩苷、小檗碱和甘草苷的肠吸收差异。葛根芩连汤中4种药效成分的浓度应用LC-MS/MS方法进行测定,血药浓度时间数据利用DAS软件进行动力学分析,并结合大鼠肠外翻及在体单向肠道灌流实验数据,比较不同配伍方式下4种成分的吸收差异。结果显示全方组中葛根素、黄芩苷及小檗碱AUC值显著提高,甘草苷AUC值显著降低,而肠外翻及在体单向肠道灌流的实验结果表明4种成分在全方组吸收明显提高,说明全方配伍可促进4种成分肠道吸收,但同时其可能加快了肝药酶对甘草苷的代谢。To analyse and compare the characteristics of the intestinal absorption of puerarin, baicalin, berberine and liquiritin in different combinations of Gegenqinlian decoction based on pharmacokinetic parameters, a sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was applied for the quantification of four components in rat's plasma. And pharmacokinetic parameters were determined from the plasma concentration-time data with the DAS software package. The influence of different combinations on pharmacokinetics of four components was studied to analyse and compare the absorption difference of four components, together with the results of the in vitro everted gut model and the rat single pass intestinal perfusion model. The results showed that compared with other combinations, the AUC values of puerarin, baicalin and berberine were increased significantly in Gegenqinlian decoction group, while the AUC value of liquiritin was reduced. Moreover, the absorption of four components was increased significantly supported by the results from the in vitro everted gut model and the rat single pass intestinal perfusion model, which indicated that the Gegenqinlian decoction may promote the absorption of four components and accelerate the metabolism of liquiritin by the cytochrome P450.

关 键 词:中药复方 药代动力学 葛根芩连汤 配伍 肠吸收 

分 类 号:R943[医药卫生—药剂学]

 

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