2011年中国北方H9N2亚型禽流感病毒分离株HA基因进化分析及受体结合性检测  

Phylogenetic analysis of the hemagglutinin genes and detection of receptor binding specificity of H9N2 influenza viruses isolated from Northern China

作　　者：屈亚锦[1] 屈秋锦 李超 刘月月[1] 石迎[1] 刘立涛[1] 任庆海[1] 刘思当[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东省医疗器械产品质量检验中心,山东济南250101 [3]夏津县畜牧兽医局,山东夏津253200

出　　处：《中国预防兽医学报》2013年第10期787-790,816,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31172296)

摘　　要：为了解近期中国北方禽源H9N2亚型禽流感病毒(AIV)流行规律及分子遗传进化特征,本实验对2011年在中国北方家禽中分离到的11株H9N2亚型AIV通过RT-PCR扩增病毒的HA基因片段,进行测序及遗传进化分析,并对这些病毒的受体结合性进行了检测。结果表明:11株H9N2亚型AIV分离株的HA基因在HA1和HA2的氨基酸裂解位点均为PSRSSR/GLF基序,符合低致病性病毒株氨基酸序列特征。多数病毒HA潜在糖基化位点为8个,所有分离株病毒的受体结合位点226位均为L,经红细胞受体结合性试验验证表明这些病毒均同时具有α-2,3和α-2,6受体结合特性,表明目前北方地区流行的H9N2亚型AIV具有感染哺乳动物的潜在威胁。本研究结果对加强H9N2病毒的分子流行病学监测具有重要意义。To study the evolutionary characteristics of H9N2 avian influenza virus （AIV） isolated from avian in Northern China, the hemagglutinin （HA） gene of eleven H9N2 AIVs isolated since 2011 were analyzed and their receptor binding specificity were tested by a modified CRBC HA assay. The results showed that the deduced amino acid sequences of cleavage sites in the eleven H9N2 isolates were typical low pathogenic motif （PSRSSR）. Most of the isolates had eight potential glycosylation sites and all the isolates had an amino acid residue of leucine （L） at position 226 （H3 numbering）. In addition, these isolates were able to binding with both the SAcx2, 3Gal and SAcx2, 6Gal, which suggested that these viruses posed a threat to public and veterinary health. The continuing evolution of H9N2 influenza viruses in China emphasizes the importance of H9N2 influenza virus surveillance throughout this region.

关 键 词：禽流感病毒 H9N2亚型 HA基因 进化分析 受体结合性 

分 类 号：S852.65[农业科学—基础兽医学]