TLM钛合金表面白蛋白涂层的制备及抗凝血性研究  

Preparation and Anticoagulant Property of TLM Titanium Alloy with Surface Modification by Albumin-immobilization

作　　者：刘春潮[1] 于振涛[1] 余森[1] 韩建业[1] 牛金龙[1] 

机构地区：[1]西北有色金属研究院,陕西西安710016

出　　处：《钛工业进展》2013年第5期26-30,共5页Titanium Industry Progress

基　　金：国家科技支撑计划(2012BAI18B02);国家自然科学基金(31100693/C100302);国家973计划(2012CB619102);陕西省工业攻关(2012K07-03)

摘　　要：为提高医用钛合金Ti-3Zr-2Sn-3Mo-25Nb的抗凝血性能,首先通过溶胶-凝胶法在其表面制备一层TiO2薄膜,再将该TiO2薄膜活化处理,最后通过静电自组装法将牛血清白蛋白固定在TiO2薄膜表面形成抗凝血涂层。利用X射线衍射仪(XRD)、扫描电子显微镜(SEM)和接触角测试仪研究了该抗凝血涂层的相结构、表面微观形貌和亲水性特征,并通过动态凝血时间法和血小板黏附实验对比研究了白蛋白修饰前后Ti-3Zr-2Sn-3Mo-25Nb合金的抗凝血性能。结果表明,经白蛋白表面修饰处理后,Ti-3Zr-2Sn-3Mo-25Nb合金的抗凝血性能得到了明显的改善。In order to improve the anticoagulation and haemocompatibility of Ti-3Zr-2Sn-3Mo-25Nb alloy, a layer of TiO2 film was prepared by sol-gel method on the surface, then the coated samples were treated by hydroxyl solution and amination solution in order to introduce the OH- and NH2 - active groups, the albumin was linked on the surface of TiO2 film through the active amidine group by the electrostatic self-assembly multilayer method finally. The phase identification and surface characteristics of the coatings were successively performed and tested by XRD and SEM. The he- mocompatibility of the Ti-3Zr-2Sn-3Mo-25Nb alloy in vitro with and without albumin-immobilization were evaluated by clotting time measurement test and platelet adhesion test, and the results showed that the anticoagulation and haemo- compatibility of the Ti-3Zr-2Sn-3Mo-25Nb alloy could be remarkably improved by surface modification via albumin-immobilization.

关 键 词：Ti-3Zr-2Sn-3Mo-25Nb合金 抗凝血 白蛋白 表面改性 血液相容性 

分 类 号：R318.08[医药卫生—生物医学工程]