抗小麦黄矮病相关蛋白激酶TiDPK1与BYDV外壳蛋白的互作  

Interaction between Wheat Resistance-related Kinase TiDPK1 and BYDV Coat Protein

作　　者：汪信东[1] 陈亮[1] 张增艳[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部麦类生物学与遗传育种重点实验室,北京100081

出　　处：《作物学报》2013年第10期1720-1726,共7页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2012AA10A309;2006AA100102)资助

摘　　要：小麦黄矮病是由大麦黄矮病毒(Barley yellow dwarf virus,BYDV)引起的小麦重要病毒病。分离于小麦–中间偃麦草易位系的蛋白激酶编码基因TiDPK1,是一个抗小麦黄矮病相关基因。本文报道利用酵母双杂交技术和双分子荧光互补技术对TiDPK1与BYDV外壳蛋白(coat protein,CP)互作的研究结果。酵母双杂交分析结果表明,TiDPK1能够与BYDV-GAV、-PAV株系的CP互作,双分子荧光互补分析结果进一步表明,TiDPK1可与BYDV的CP互作、产生双分子荧光互补信号,说明TiDPK1确可与BYDV CP相互作用,该结果对了解TiDPK1在小麦抗BYDV反应机制具有一定意义。Yellow dwarf virus disease is one of the important diseases of wheat（Tritium aestivum L.）worldwide.It is caused by Barley yellow dwarf virus（BYDV）that is vectored by aphids.A kinase protein encoding gene TiDPK1,which is derived from Thinopyrum intermedium,is an important gene involved in BYDV resistance in wheat-T.intermedium translocation lines.In this study,we used yeast two-hybrid and bimolecular fluorescence complementation assays to explore the relationship between TiDPK1 and coat protein（CP）of BYDV（BYDV-CP）.The results proved that the protein TiDPK1 interacted with BYDV-CP,which may offer an insight to the resistance mechanism of TiDPK1.

关 键 词：小麦 蛋白激酶TiDPK1 大麦黄矮病毒外壳蛋白 酵母双杂交 双分子荧光互补 蛋白互作 

分 类 号：S512.1[农业科学—作物学]