刺榆中黄酮含量测定及其抗氧化活性研究  

Study on quality evaluation of different parts from Hemiptelea davidii and its antioxidant activity

作　　者：马斐斐[1] 石利春[1] 包海鹰[1] 

机构地区：[1]吉林农业大学,吉林长春130118

出　　处：《人参研究》2013年第3期19-23,共5页Ginseng Research

基　　金：吉林省科技厅"吉林省人参食品产业专利战略研究";项目编号:2011-1615

摘　　要：目的研究刺榆各部位黄酮类化合物的含量,以及其粗多糖及总黄酮的抗氧化活性。方法利用NaNO2-Al(NO3)3-NaOH显色法和可见分光光度法测定总黄酮的含量。并对总黄酮和粗多糖的抗DPPH、OH、O2-的活性进行了研究。结果总黄酮含量次序为:刺>花>叶>茎>根。刺榆黄酮(HDF)和多糖(HDP)均具有很好的抗氧化作用。结论刺榆的刺可发展为提供黄酮成分的新资源并显示显著的抗氧化活性。Objective Flavonoids contents in different parts of H.davidii were study, and the antioxidant activity of flavonoids and polysaccharide was assessed. Method The flavonoids contents were determined using the method of NaNO2-Al（NO3）3-NaOH color method and spectrophotometry determination method. And the antioxidant activity were assessed through resistance to DPPH, OH, O2-. Result The flavonoids contents were in order： thorn 〉 flower〉 leaf 〉 stem 〉 root. The total flavonoids（HDF） and polysaccharide （HDP） all has the very good antioxidant effect. Conclusion The thorns in H. davidii can be as providing flavonoids resources and has obvious antioxidant activity.

关 键 词：含量测定 抗氧化 黄酮 多糖 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]