厌氧条件下Shewanella oneidensis MR-1对2，4-二硝基甲苯的还原转化  

Reductive biotransformation of 2,4-dinitrotoluene by Shewanella oneidensis MR-1 under anaerobic conditions

作　　者：黄杰勋[1] 陈星[1] 李非里[1] 盛光遥[2] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032 [2]同济大学污染控制与资源化研究国家重点实验室环境科学与工程学院,上海200029

出　　处：《微生物学通报》2013年第9期1734-1741,共8页Microbiology China

基　　金：浙江省教育厅科研计划项目(No.Y201224801)

摘　　要：【目的】研究Shewanella oneidensis MR-1厌氧生物转化2,4-二硝基甲苯(2,4-DNT)的能力、转化过程和影响因素。【方法】以乳酸钠为电子供体,2,4-DNT为电子受体,S.oneidensis MR-1为降解菌,黄素为胞外电子载体,设立四个不同的对照体系并监测各体系在转化过程中2,4-DNT及其产物的动态变化。同时研究不同2,4-DNT浓度下细胞的生长情况,以及不同黄素浓度下2,4-DNT的降解情况。【结果】S.oneidensis MR-1菌能够高效还原转化2,4-DNT为4-氨基-2-硝基甲苯(4A2NT)和2-氨基-4-硝基甲苯(2A4NT),并将其进一步还原为2,4-二氨基甲苯(2,4-DAT),黄素能加速转化过程。【结论】S.oneidensis MR-1菌具备高效还原转化2,4-DNT的能力,为实际环境中硝基苯污染的原位修复提供科学依据。[Objective] Reductive biotransformation of 2,4-dinitrotoluene （2,4-DNT） by Shewanella oneidensis MR-1 was evaluated under anaerobic conditions. [Methods] Four systems were set up where 2,4-dinitrotoluene （2,4-DNT）, S. oneidensis MR-1 and flavins were used as electron acceptor, degrading bacteria and extracellular mediator, respectively. Concentrations of 2,4-DNT and its metabolites were monitored by time temporal sampling. Growth of S. oneidensis MR-1 under different concentrations of 2,4-DNT and biotransformation of 2,4-DNT under different concentrations of flavins were also studied. [Results] S. oneidensis MR-1 could efficiently biotransform 2,4-DNT to 4-amino-2-nitrotoluene （4A2NT） or 2-amino-4-nitrotoluene （2A4NT）, both of them were further reduced to 2,4-diaminotoluene （2,4-DAT）. The presence of flavins could accelerate the biotransformation. [Conclusion] S. oneidensis MR-1 showed a high capacity for biotransforming 2,4-DNT and could be further applied in the in situ bioremediation of nitrobenzene contaminations in the environment.

关 键 词：SHEWANELLA oneidensis MR-1 2 4-二硝基甲苯 厌氧还原 生物转化 

分 类 号：X50[环境科学与工程—环境工程]