奈韦拉平与铬天青S的电荷转移反应及其测定  

Determination of nevirapineoride based on the charge transfer reaction between nevirapineoride and Chromeazurol S

作　　者：李华侃[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001

出　　处：《化学研究与应用》2013年第9期1283-1285,共3页Chemical Research and Application

摘　　要：确定了奈韦拉平与铬天青S的电荷转移反应条件,同时建立了测定奈韦拉平的分光光度法。奈韦拉平与铬天青S在水和丙酮混合溶剂中发生电荷转移反应,常温下反应30min后可比色。电荷转移络合物的最大吸收波长是578nm,表观摩尔吸光系数为1.78×104L·mol-1·cm-1。奈韦拉平质量浓度在1～16μg·mL-1范围内服从比耳定律,相关系数为0.9993。当奈韦拉平浓度为6μg·mL-1时,六次测定结果的相对标准偏差为1.61%。测得荷移络合物的组成和稳定常数分别为1∶1和1.73×104。本方法用于测定制剂中奈韦拉平的含量,结果与文献方法一致,回收率在98.0%以上。The charge transfer reaction in water-acetone between nevirapineoride as donor and chromeazurol S as acceptor was investigated by spectrephotometry. The experimental optimum conditions were established. The apparent molar absorptivity of charge transfer complex is 1.78×10^4L.mol^-1 .cm^-1 at 578 nm. Beer＇ s Law is obeyed in the range of 1 - 16μg.mL^-1 of nevirapineoride with correlation ceeffcient of 0. 9993. The relative standard deviation is 1.61% （n = 6）when the concentration of nevirapineoride is 6μg-mL-1. The average recovery was over 98.0%. The composition ratio and stability constant is 1：1 and 1.73×10^4of the charge transfer complex in water-acetone between nevirapineoride and chromeazurol S respectively. The proposed method had been applied to determine the content of nevirapineoride in preparations with satisfactory results.

关 键 词：奈韦拉平 铬天青S 电荷转移反应 

分 类 号：O657.32[理学—分析化学]