甲基紫探针分光光度法测定利福平  

Spectrophotometric determination of rifampicin with methyl violet as probe

作　　者：江虹[1] 刘艳[1] 庞向东[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《化学研究与应用》2013年第9期1286-1289,共4页Chemical Research and Application

基　　金：重庆市教委科技基金项目(KJ121321)资助

摘　　要：在pH 7.3的Tris-HCl缓冲溶液中,甲基紫与利福平反应生成具有正吸收峰和负吸收峰的紫色络合物。其最大正吸收波长位于500 nm,最大和次大负吸收波长分别位于540 nm和605 nm,利福平的质量浓度在0.2～0.82 mg·L-1(正吸收)和0.0～0.82 mg·L-1(负吸收)以内与吸光度A呈线性关系,服从比尔定律,表观摩尔吸光系数分别为8.90×104(500 nm)、4.41×105(540 nm)和3.04×105(605 nm)L·mol-1·cm-1,该法用于市售利福平药物中利福平的测定结果满意。In pH 7.3 Tris-HCl buffer medium, methyl violet reacted with rifampicin to form purple complexes with an obvious plus absorption peak and two fading peak. The maximum plus absorption wavelength was at 500 nm. The maximum negative absorption wavelength and sub-major negative absorption wavelength were 540 nm and 605 nm respectively. Absorbency was linearly propertional to th.e concentration of rifampicin in a range of 0. 2- 0. 82 mg.L^-1（ plus absorption）and 0. 0 -0. 82 mg/L（ negative absorption） at the absorption wavelength of 500 or 540 or 605 nm and rifampiein obey Beer＇s law in definite concentration range. The apparent molar absorptivity（ε）were 8.90×10^4（500 nm）and 4.41×10^5（540 nm） and 3.04×10^5（605 nm） L.mol. cm^-1 respectively. The method was applied to the determination of rifampicin in the city sell rifampicin medicament with satisfactory results.

关 键 词：甲基紫 利福平 分光光度法 

分 类 号：O657.32[理学—分析化学]