对2010年版《中国药典》山楂叶中总黄酮含量测定方法的商榷  

Discussion about the Method for Content Determination of Flavonoids in Crataegi Folium Stated in 2010 Edition of Chinese Pharmacopoeia

作　　者：王肖[1] 吴国成 李赛颖 沈莹 李艳春 潘海峰[1] 

机构地区：[1]承德医学院河北省中药研究与开发重点实验室,河北承德067000 [2]承德御室金丹药业有限公司,河北承德067000

出　　处：《中国药房》2013年第35期3309-3311,共3页China Pharmacy

基　　金：承德市科学技术研究与发展计划项目(No.20101312)

摘　　要：目的:探讨2010年版《中国药典》山楂叶中总黄酮含量的测定方法。方法:以芦丁为对照品,用紫外-可见分光光度法测定山楂叶中总黄酮的含量,并结合高效液相色谱法考察山楂叶中总黄酮含量测定方法的准确性。结果:B环无邻二酚羟基结构的黄酮类化合物,采用芦丁显色紫外-可见分光光度法测定总黄酮含量误差较大,也不是总黄酮含量测定的专属方法。结论:芦丁显色紫外-可见分光光度法测定山楂叶中总黄酮含量的方法值得商榷,建议将芦丁显色法和高效液相色谱法结合起来测定,这样会更科学、准确。OBJECTIVE：To discuss the method for the content determination of flavonoids in Crataegi Folium in Chinese Pharmacopoeia（2010 edition）.METHODS：Using rutin as control,the content of flavonoids in Crataegi Folium was determined by ultraviolet-visible spectrophotometry,and the accuracy of the method was investigated by combined with HPLC.RESULTS：There were biggish error to determine the flavonoids content of structure without adjacent diphenol hydroxyl in B benzene ring by the ultraviolet-visible spectrophotometry,and it was also not the exclusive method.CONCLUSIONS：It is questionable to determine the content of total flavonoids in Crataegi Folium by the rutin colorimetric ultraviolet-visible spectrophotometry.So we suggest to use the method of rutin colorimetric method combined with HPLC.It is more scientific and accurate.

关 键 词：山楂叶 芦丁 总黄酮 紫外-可见分光光度法 高效液相色谱法 中国药典 商榷 

分 类 号：R284.1[医药卫生—中药学]