小麦锈病活体筛选方法的规范化  

Wheat Rust in vivo Screening Method Standardization

作　　者：王满意[1] 于淑晶[2] 张晓[2] 王力钟[2] 赵卫光[1] 李宝聚[3] 

机构地区：[1]南开大学农药国家工程研究中心,天津300071 [2]南开大学元素有机化学研究所,天津300071 [3]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《农药》2013年第9期677-679,683,共4页Agrochemicals

基　　金："十二五"国家科技支撑计划课题研究内容(2011BAE06B08-04)

摘　　要：【目的】为规范小麦锈病活体筛选方法，对接种方法、培养条件进行系统的筛选和比较，并利用3种标准药剂进行验证。【结果】小麦叶锈病最佳接菌浓度为1×10^8-5×10^8cfu／L，最佳接菌方法为喷雾法；20℃黑暗保湿培养24h为最佳侵入条件；20℃、24h光照培养为最佳潜育条件；3种药剂的浓度对数与防效之间呈现出很好的线性关系。【结论】规范后的小麦锈病室内活体筛选方法准确可行。[Aims] In order to standardize wheat rust screening method, different inoculation methods and culture conditions were screened and compared systematically, and 3 standard chemicals were verified by screening. [Results] The results showed that the best way for wheat rust screening method is spraying spores suspension on wheat leaves with spores concentration range from 1×10^8 to 5×10^8cfu/L, retention in moisture and darkness for 24 h at 20 ℃, and then in moisture and light for 24 h at 20℃ until full onset. There is a good linear relationship between the concentration logarithm and fungicide control effect of the 3 standard chemicals. [Conclusions] The wheat rust screening method standardized has good accuracy.

关 键 词：小麦锈病 筛选 规范化 

分 类 号：TQ455.4[化学工程—农药化工]