滋阴益气活血解毒法对糖尿病合并脂肪肝小鼠SREBP-1c表达的影响  

Influence of Ziyin Yiqi Huoxue Jiedu Fang on SREBP-1c expression in mice with diabetes complicating fatty liver

作　　者：成细华[1] 程莉娟[1] 张琴[1] 曾幸[1] 喻嵘[1] 

机构地区：[1]湖南中医药大学,湖南410208

出　　处：《北京中医药大学学报》2013年第8期534-537,I0001,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(No.81102593);中国博士后科学基金(No.20110491258);教育部博士学科点科研基金(No.20104323110002;20094323120001);湖南省教育厅优秀青年项目(No.11B091);湖南省科技厅博士后科学基金(No.2011RS4017);湖南省科技计划一般项目(No.2012FJ4096);湖南省大学生研究性学习和创新性实验计划项目(No.2012-185);地方高校国家级大学生创新创业训练计划项目(No.201210541003);湖南省重点学科中西医结合基础和中医内科学资助

摘　　要：目的探讨滋阴益气活血解毒方对高脂饮食2型糖尿病转基因MKR小鼠脂肪肝发生中固醇调节元件结合蛋白-1c(SREBP-1c)表达的影响。方法 30只MKR小鼠随机分为对照组、高脂模型组和滋阴益气活血解毒方(中药方)组;高脂模型组和中药方组均以高脂饲料喂养8周,对照组以基础饲料喂养;中药方组高脂饲料喂养8周后,以中药含生药量29.64 g/kg灌胃干预治疗4周,其他2组灌胃等量的蒸馏水;光镜下观察小鼠肝组织脂肪变性,RT-PCR检测肝组织SREBP-1c mRNA表达,免疫组化检测肝组织SREBP-1c蛋白表达。结果高脂模型组小鼠较对照组小鼠肝脏SREBP-1c mRNA和蛋白表达增加,差异具有统计学意义(P<0.01);中药方组降低高脂饮食MKR小鼠肝脏SREBP-1c的mRNA和蛋白表达水平,与高脂模型组比较差异具有统计学意义(P<0.01)。结论滋阴益气活血解毒方通过调节肝脏SREBP-1c表达,缓解糖尿病合并脂肪肝小鼠肝脏脂肪性变。Objective To investigate the influence of Ziyin Yiqi Huoxue Jiedu Fang on SREBP-1c expression in transgenic MKR mice with type 2 diabetes complicatiing fatty liver induced by high fat diet.Methods MKR mice （n =30） were randomly divided into control group,model group and Ziyin Yiqi Huoxue Jiedu（Ziyin group）.The model group and Ziyin group were fed with high fat feeds and control group with basic feeds for 8 weeks.After 8 weeks Ziyin group was intragastrically given Chinese medicinal （crude medicinal,29.64 g/kg） for 4 weeks.Other two groups were given distilled water.The hepatic steatosis was observed by using light microscope.The expression of SREBP-1c mRNA was detected by using reverse transcription polymerase chain reaction （RT-PCR） and expression of SREBP-1 c protein was detected by using immunohistochemistry technique.Results The expressions of SREBP-1c mRNA and SREBP-1c protein increased in model group compared with control group （P ＜0.01）.The expressions of SREBP-1c mRNA and SREBP-1 c protein decreased in Ziyin group,and difference in the expression of SREBP-1c mRNA had statistical significance between Ziyin group and model group （P ＜0.01）.Conclusion Ziyin Yiqi Huoxue Jiedu Fang can relieve the hepatic steatosis through regulating the expression of SREBP-1c in mice with diabetes complicating fatty liver.

关 键 词：滋阴益气活血解毒方 糖尿病合并脂肪肝 固醇调节元件结合蛋白-1C MKR小鼠 

分 类 号：R285.5[医药卫生—中药学]