参七消痞颗粒中野黄芩苷和丹酚酸B含量测定方法研究  

Quantitative determination of scutellarin and salvianolic acid B in Shenqi Xiaopi Granules

作　　者：张乐[1] 张强[1] 刘娟[1] 孟祥娟[1] 刘斌[1] 姜艳艳[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2013年第8期550-553,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重大科技专项新药创制项目(No.2010ZX09102-211)

摘　　要：目的 建立参七消痞颗粒中野黄芩苷和丹酚酸B的含量测定方法.方法 采用HPLC法,色谱柱为Waters SunFireTM C18（4.6×150 mm,5μm）,流动相为甲醇-0.1％磷酸水溶液梯度洗脱,流速为1.0 mL/min,柱温为30℃,野黄芩苷和丹酚酸B检测波长分别为335 nm、280 nm.结果 野黄芩苷和丹酚酸B分别在0.064 16 ～0.385 0μg和0.743 ～4.455 μg质量范围内与色谱峰面积呈良好线性关系,相关系数分别为0.999 5和0.999 4.野黄芩苷和丹酚酸B的平均加样回收率分别为101.9％和96.3％,RSD分别为1.17％和0.33％.结论 本方法简单可靠,结果准确,可用于参七消痞颗粒的质量控制.Objective To establish a method for quantitative determination of scutellarin and salvianolic acid B in Shenqi Xiaopi Granules.Methods The HPLC procedure was performed on the chromatographic column of Waters SunFireTM C18 （4.6 x 150 mm,5 μm） at 30℃,and the mobile phase was methanol-0.1％ phosphoric acid solution in gradient elution.The flow velocity was 1.0 mL/min.The detection wavelength of scutellarin was 335 nm,and that of salvianolic acid B was 280 nm.Results Scutellarin and salvianolic acid B respectively showed a good linear relationship with peak area at a range from 0.064 16 μg to 0.385 0 μg and from 0.743 μg to 4.455 μg,and the correlation coefficients were 0.999 5 and 0.999 4.The average recovery of scutellarin was 101.9 ％ （RSD =1.17％）,and that of salvianolic acid B was 96.3 ％ （RSD =0.33％）.Conclusion The method is simple,reliable and accurate,which can be used for the quality control of Shenqi Xiaopi Granules.

关 键 词：野黄芩苷 丹酚酸B 参七消痞颗粒 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]