清热消积方对SPC-A-1肺癌细胞及其诱导的HUVEC内皮细胞表达VEGF、bFGF的影响  

Expressions of VEGF and bFGF on Human Umbilical Vein Endothelial Cell Induced by Human Lung Adenocarcinoma Cells Treated with Qingre Xiaoji Recipe

作　　者：陈培丰[1] 金莹祺[1] 潘磊[1] 

机构地区：[1]浙江中医药大学附属第一医院肿瘤科,浙江杭州310006

出　　处：《中华中医药学刊》2013年第9期1836-1838,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教育厅重点资助项目(Z2009-07128)

摘　　要：目的:研究清热消积方对SPC-A-1肺癌细胞及其诱导的HUVEC内皮细胞表达VEGF、bFGF的影响,并探讨其抗肿瘤血管生成的部分作用机制。方法:以不同浓度的清热消积方药液干预SPC-A-1肺癌细胞及其诱导的HUVEC内皮细胞后,采用双抗体夹心ELISA法分别检测两种细胞上清液中VEGF、bFGF的表达量。结果:经清热消积方药液干预后,该两种细胞的VEGF、bFGF表达量均出现不同程度的下降,且与药物浓度呈负相关,具有良好的浓度依赖性(P<0.05)。结论:清热消积方抑制SPC-A-1肺癌细胞及其诱导的血管内皮细胞表达VEGF、bFGF,可能是其抗肿瘤血管生成的作用机制之一。Objective ：To investigate the expressions of VEGF and bFGF on human umbilical vein endothelial cell in- duced by human lung adenocarcinoma cells which were treated with Qingre Xiaoji Recipe, and to explore the part mecha- nism of anti - tumor angiogenesis effect of it. Methods ： Different concentrations of Qingre Xiaoji Recipe were employed to affect HUVEC which was induced by SPC - A - 1. Then ELISA method was applied to detect the expressions of VEGF and bFGF in supernatant from the two kinds of cells. Results： The expressions of VEGF and hFGF in the two groups showed different degrees of decline, and there was a negative correlation with concentration of drug, and represented good concentration dependence （ P 〈 0.05 ）. Conclusion ： Qingre Xianji Recipe could inhibit the expressions of VEGF and bF- GF on human umbilical vein endothelial cells which were induced by human lung adenocarcinoma cells, and it might be one of the mechanisms on anti - tumor angiogenesis effects.

关 键 词：清热消积方 SPC—A-1 HUVEC VEGF BFGF 

分 类 号：R285.5[医药卫生—中药学]