右归饮协同干细胞转染VEGF移植修复激素性骨坏死的研究  

Experimental Study of Youguiyin Combined with Mesenchymal Stem Cells Transfected with VEGF Transplantation for Repairing Steroid-induced Avascular Necrosis of Femoral Head

作　　者：王萧枫 许兵 童培建[2] 金红婷[2] 肖鲁伟[2] 

机构地区：[1]温州市中西医结合医院,浙江温州325000 [2]浙江中医药大学骨伤研究所,浙江杭州310053

出　　处：《中华中医药学刊》2013年第9期1930-1933,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省卫生厅资助项目(2009A197);浙江省中医药管理局资助项目(2009YA021);温州市科技局资助项目(Y20090039)

摘　　要：目的:观察右归饮协同骨髓干细胞(Mesenchymal Stem Cells,MSCs)转染VEGF(Vascular Endothelial Growth Factor,血管内皮细胞生长因子)移植对激素性股骨头坏死家兔模型骨修复的影响。方法:45只兔随机分为5组:A、B、C、D组为模型组,醋酸泼尼松6周注射建立兔SANFH模型,E组为空白对照组;分离、培养、增殖MSCs,并利用慢病毒载体对MSCs进行VEGF165基因转染;B组进行髓芯减压+MSCs移植,C组进行髓芯减压+VEGF转染MSCs移植,D组进行右归饮灌胃治疗协同髓芯减压+VEGF转染MSCs移植,细胞移植量均1×106。对第8、10、12周的各组一般形态学、血脂变化、股骨头组织病理学和RT-PCR观察VEGF表达情况进行检测。结果:B、C、D组较A组的观测指标均有明显改善,其中血脂指标、空骨陷窝阳性率和VEGF阳性表达各组与A组组间比较差异均有统计学意义(P<0.01),且D组改善优于B、C两组(P<0.05)。结论:右归饮协同转染VEGF的MSCs移植治疗SANFH中能发挥更好的疗效,有较好的改善血运与骨修复重建作用。Objective ：To observe the effect of Youguiyin combined with mesenchymal stem cells （MSCs） transfected with VEGF （vascular endothelial growth factor） transplantation for repairing rabbit model of steroid - induced avascular necrosis of femoral head（SANFH）. Methods： 45 rabbits were randomly divided into 5 groups： group A, B, C, D were SANFH rabbit model which were established by six weeks injection of prednisone acetate. Group E was the control group; rabbit MSCs were isolated and cultured and then transfected with VEGF165 gene by lentiviral vector（LV）. The core de- compression with MSCs transplantation was on group B, core decompression with hVEGF165 - MSCs transplantation on group C,and group D added Youguiyin consecutive treatment with hVEGF165 -MSCs transplantation therapy. The cell transplantation volume was 1 x 106. The general morphology of each group of 8,10,12 weeks, blood lipid changes, histo- pathology of femoral head and RT - PCR were observed and the expression of VEGF were detected. Results ： Compared with group A, Group B, C,D showed significant improvement in lipid parameters, positive rates of empty lacunae, and VEGF expression and each group and group A had statistically significant differences（ P 〈 O. 01 ）, and group D was superior to group B and C（P 〈0.05）. Conclusion：Collaborative transplantation of hVEGF165 -MSCs with Youguiyin can get better outcomes in treating SANFH, better improving the blood supply and bone repair and reconstruction role.

关 键 词：激素性股骨头坏死 血管内皮细胞生长因子 基因转染 骨髓基质干细胞 右归饮 

分 类 号：R295.5[医药卫生—民族医学]