多倍体与八倍体半夏中草酸钙晶体含量的测定  

Content Determination of Calcium Oxalate in Octoploid and Polyploid Pinellia

作　　者：潘平[1] 李伟平[1] 鲁光耀[1] 吕迪[1] 蒋福升[1] 丁志山[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2013年第9期1985-1987,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2013AZ031);浙江省大学生科技成果推广项目(2010R410041)

摘　　要：目的:测定多倍体(十六倍体)半夏和八倍体半夏原球茎以及块茎中草酸钙晶体的含量。方法:采用高效液相色谱法,以0.5%磷酸水溶液为流动相,以草酸为对照品,测定多倍体半夏和八倍体半夏中草酸钙晶体的含量。结果:多倍体半夏原球茎和块茎中草酸钙晶体的含量分别为(2.799±0.017)%和(1.375±0.037)%,八倍体半夏原球茎和块茎中草酸钙晶体含量分为为(1.136±0.028)%和(1.924±0.05)%。结论:半夏原球茎和块茎中均含有较高含量的草酸钙晶体,与八倍体半夏相比,多倍体半夏块茎中的草酸钙晶体明显比八倍体半夏低。Objective：To determine the content of Calcium Oxalate in protocorm and tuber of octoploid and polyploid （hexadecaploid） Pinellia. Methods ： To determine the content of Calcium Oxalate in octoploid and polyploid Pinellia by HPLC method with O. 5% Phosphoric Acid as mobile phase and oxalic acid as standard preparation. Results：The content of Calcium Oxalate in protocorm of polyploid Pinellia was （2. 799 ± 0.017）% while （1. 375 ±0. 037）% in tubers,there were （ 1. 136 ± 0. 028） % Calcium Oxalate in protocorm of octoploid Pinellia while （ 1. 924 ± 0.05 ） % in tubers. Conclu- sion ： There is high content of Calcium Oxalate in protocorm and tuber of Pinellia, compared with octoploid Pinellia, the tu- bers of polyploid Pinellia has lowest content of Calcium Oxalate.

关 键 词：半夏 草酸钙晶体 多倍体 八倍体 HPLC 

分 类 号：R284.1[医药卫生—中药学]