丹参酮ⅡA对压力负荷增加大鼠心肌CTGF表达的影响  

Expression of Myocardial CTGF in Rats with Pressure Overload and Effect of Tanshinone ⅡA

作　　者：常文静[1] 赵凌杰[1] 赵智明[1] 沈思钰[1] 董晓蕾[1] 蔡辉[1] 

机构地区：[1]南京军区南京总医院中西医结合科,江苏南京210002

出　　处：《中华中医药学刊》2013年第9期2013-2016,I0010,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：南京总医院科研基金面上项目(2010Q027)

摘　　要：目的:观察压力负荷增加大鼠心肌组织结缔组织生长因子(Connective Tissue Growth Factor,CTGF)表达的改变,探讨丹参酮ⅡA(TanshinoneⅡA,TanⅡA)对心肌纤维化的保护作用。方法:采用腹主动脉缩窄术制备压力负荷增加心肌纤维化模型,随机分成4组(n=8),假手术组(Sham组)、模型组(Model组)、丹参酮ⅡA组[TanⅡA组,20 mg/(kg·d)]及阳性对照药物卡托普利组[Captopril组,100 mg/(kg·d)]。术后4周成功造模并开始给药,疗程为4周。8周后检测心室质量指数、左心室重量指数、心肌组织病理学、心肌羟脯氨酸含量以及心肌组织CTGF蛋白含量。结果:①与Sham组比较,Model组大鼠心脏质量指数和左心脏质量指数明显升高(P<0.01);与Model组比较,TanⅡA组则显著降低(P<0.01)。②与Sham组比较,Model组大鼠心肌羟脯氨酸含量明显升高(P<0.01);与Model组比较,TanⅡA组则显著降低(P<0.01)。③与Sham组比较,Model组大鼠心肌组织CTGF表达显著升高(P<0.01);与Model组比较,TanⅡA组则显著降低(P<0.01)。结论:TanⅡA组能抑制心肌肥厚,减轻心肌纤维化,此作用可能与下调心肌局部CTGF水平有关。Objective：To observe the changes of connective tissue growth factor （CTGF） expression in rat myocardial tissue with pressure overload, and to explore the protective effects of Tanshinone II A （Tan II A） on myocardial fibrosis. Methods ： Twenty - four Sprague - Dawley （ SD ） rats induced by the abdominal aorta constriction preparation were random- ly divided into following apporches （n = 8） ： model group （Model group）, Tan II A group [ Tan I A group 20 mg（/kg · d） ] and positive captopril group[ captopril group 100 mg/（kg d） ]. The matched age SD sham operation （n =8） was as control. The model of pressure overload - induced myocardial fibrosis was successfully established after 4 weeks of opera- tion. After four weeks of Tan II A treatment, cardiac mass index, left ventricular mass index, histopathological, myocar- dial hydroxyproline content, myocardial CTGF expression were measured. Results ： （!）Compared with sham group, cardiac mass index and left ventricular mass index were increased in Model group （P 〈 0.01 ） ; Compared with Model group, these indexes were decreased in Tan II A group （P 〈 0.01 ）. （2）Compared with sham group, myocardial hydroxyproline content was increased in Model group （P 〈 0.01 ） ; Compared with Model group, Tan II A had significantly reduced myo- Cardial hydroxyproline content （P 〈 0.01 ）. （3）Compared with sham group, CTGF protein content of myocardial tissue was increased in Model group （P 〈 0.01 ） ; Compared with Model group, Tan II A had significantly reduced the expression of CTGF. Conclusions ： Tan II A can inhibit cardiac hypertrophy, attenuate myocardial fibrosis, and this effect may be associ- ated with down-regulation of the expression of fibrogenic cytokines CTGF.

关 键 词：压力负荷 心肌纤维化 结缔组织生长因子 丹参酮ⅡA 

分 类 号：R332[医药卫生—人体生理学]