异源四倍体鲫鲤肝组织线粒体DNA的研究  

Study on the mtDNA from liver of allotetraploid hybrids of red crucian carp(♀) × common carp(♂)

作　　者：黎双飞[1] 刘少军[1] 张轩杰[1] 罗琛[1] 周工建[1] 刘筠[1] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《水产学报》2000年第6期489-43,共1页Journal of Fisheries of China

基　　金：湖南省自然科学基金重点资助项目!(湘科技字199737)

摘　　要：用密度梯度离心和ＤＮａｓｅ　Ⅰ、ＲＮａｓｅ消化法从异源四倍体鲫鲤（Ｆ８）和鲫鲤Ｆ２肝组织中提取和纯化线粒体ＤＮＡ（ｍｔＤＮＡ）。用９种限制性内切酶对异源四倍体鲫鲤和鲫鲤Ｆ２的ｍｔＤＮＡ进行了分析，结果表明ＸｂａⅠ和ＢｇｌⅡ两种限制性内切酶在异源四倍体鲫鲤ｍｔＤＮＡ上存在酶切位点多态性。通过凝胶电泳测得异源四倍体鲫鲤ｍｔＤＮＡ相对分子量平均约 １０．２９ × １０６道尔顿，分子大小为 １６．２０ｋｂ；鲫鲤 Ｆ２ ｍｔＤＮＡ相对分子量为 １０． １８ × １０６道尔顿，分子大小为１６．０２ｋｂ。根据单酶解和双酶解的片段数目和分子大小，构建了异源四倍体鲫鲤ｍｔＤＮＡ的７种限制性内切酶（Ｋｐｎ　Ⅰ、Ｐｓｔ、ＳａｌⅠ、ＸｈｏⅠ、Ｂｇｌ Ⅱ、ＢａｍＨⅠ和 ＸｂａⅠ）酶切图谱。The mitochondrial DNA(mtDNA) from liver of allotetraploid F8 hybrids of red crucian carp(Carassius auratus red var. )( ) x common Carp(Cyprinus carpio L. )( ) and from liver of F2 hybrids were respectively isolated by means of the density gradient centrifugation and nuclease digestion, and analyses by 9 kinds of restriction endonucleases. The Xba Ⅰ and Bgl Ⅱ showed restriction site polymorphisms in the mtDNA from allotetraploid hybrids. The moleculer weight of mtDNA of allotetraploid hybrids and F2 hybrids were respectively 10.29x 106 daltons with 16.20 x 103 base pairs and 10. 18 x 106 daltons with 16.02 x 103 base pairs, the data were estimated by using agarose gel electrophoresis. Based on the number and size of the restriction fragments obtained by single and double digestion, the mtDNA of allotetraploid hybrids physical map of 7 kinds of restriction endonucleases Xba ⅠBamH ⅠKpn I.Pst Ⅰ.Sal I.Xho Ⅰ and Bgl Ⅱ was constructed.

关 键 词：异源四倍体鲫鲤 线料 DNA 限制性内酶 图谱 

分 类 号：Q959.468[生物学—动物学] Q343.35