碳量子点-VB_2荧光共振能量转移法测定VB_2  

Fluorescence Resonance Energy Transfer Between CDs and Vitamin B_2 with Its Application in Determination of Vitamin B_2

作　　者：查珺[1] 刘信[1] 王克文[1] 陈志兵[2,1] 

机构地区：[1]宿州学院化学与生命科学学院,安徽宿州市汴河路71号234000 [2]自旋电子与纳米材料安徽省重点实验室培育基地,安徽宿州市汴河路71号234000

出　　处：《光谱实验室》2013年第5期2089-2093,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金项目(21271136);安徽省教育厅重点项目(KJ2012A267);宿州学院科研平台开放课题(2012YKF01);宿州学院国家级大学生创新创业训练计划项目(201210379011)资助

摘　　要：用微波法合成了具有发光特性的CDs,以发射波长为447nm的CDs为供体,维生素B2(V B2)为受体,建立了一种以十六烷基三甲基溴化铵(CTAB)为介质的荧光共振能量体系检测VB2含量的方法。结果表明:在pH=7.1时,VB2的浓度与F a/Fd值在1.8×10-8—3.0×10-7mol/L范围内呈线性关系,其线性方程为F a/Fd=0.0766C(×10-8mol/L)+0.5658,相关系数为0.9996,检出限为8.3×10-9mol/L。The water-soluble carbon dots （CDs） was synthesized using microwave method. The fluorescence resonance energy transfer （FERT） between the CDs and Vitamin Bz （VB2） in the presence of cetyltrimethylammonium bromide （CTAB） was characterized,in which the CDs with an emission wavelength of 447 nm was used as energy donor and VBa as aeeeptor. Vitamin B2 was determined by FERT. The results showed that the extent of Fa/Fd was linearly proportional to the concentration of VB2 from 1.8×10-8-3.0×10-7mol/L with a correlation coefficient of 0. 9996. The minimum detection concentration was 8.3×10-9 mol/L.

关 键 词：维生素B2 碳量子点 荧光共振能量转移 

分 类 号：O657.32[理学—分析化学]