直接法测定普洛色林磷脂复合物的复合率  

Determination of Complexation Rate of Neostigmine Bromide-Phospholipid Complex by the Direct Method

作　　者：李嫄[1] 胡江波[1] 张敉[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心,重庆市渝中区医学院路1号400016

出　　处：《光谱实验室》2013年第5期2251-2254,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：建立普洛色林磷脂复合物复合率的测定方法。采用紫外分光光度法测定普洛色林磷脂复合物中参与复合的药物的量,由此计算出复合率。结果表明普洛色林在260nm处有最大吸收,浓度在50—500Lg/mL时与吸光度呈良好的线性关系,回归方程为:A=0.0018C+0.0109,r=0.9999(n=3);平均加标回收率为99.40%,RSD为0.54%。本方法操作简便、快捷,具有良好的准确度和精密度。The complexation rate of neostigmine bromide-phospholipid complex was determined at the wavelength of 260 nm by UV-visible spectrophotometry. The neostigmine bromide had a good liner relationship range from 50--500μg/mL-1. The regression equation was as follows.A=0. 0018C ＋ 0. 0109,r= 0. 9999 （n= 3）. The average recovery rate was 99. 40%（n= 9）, and the relative standard deviation （RSD） was 0. 54%, respectively. The method was simple,accurate and rapid, which can be used for the content determination of neostigmine bromide in neostigmine bromide- phospholipid complex.

关 键 词：普洛色林 磷脂复合物 直接法 复合率 

分 类 号：O657.32[理学—分析化学]