HPLC法测定赤芍提取物中芍药苷的含量  

Content Determination of Paeoniflorin inRadix Paeoniae Rubra Extract by HPLC

作　　者：张韻慧[1] 娄辉[1] 张旺[2] 胡文文[1] 张崧[3] 晋兴华[1] 

机构地区：[1]天津大学药物科学与技术学院,天津市南开区卫津路92号300072 [2]天津红日药业股份有限公司 [3]天津大学化工学院,天津市南开区卫津路92号300072

出　　处：《光谱实验室》2013年第5期2424-2427,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：天津市自然基金重点项目(11JCZDJC20600)

摘　　要：建立了赤芍水提物正丁醇萃取物中芍药苷的含量测定方法。采用Diamonsil C18色谱柱(4.6mm×250mm,5Lm),以甲醇(A)-超纯水(0.2%的冰醋酸)(B)(30∶70,V/V)为流动相等度洗脱,流速:1mL·min-1;柱温:30℃;检测波长:230nm;进样量:10LL。赤芍水提物正丁醇萃取物中芍药苷的平均含量为1.52%。经正丁醇萃取后,提取物中芍药苷的纯度有了较大提高。所用含量测定方法简便准确,分离度好,可用于赤芍提取物中芍药苷的含量测定。正丁醇可用于赤芍中芍药苷的提取纯化工艺。The method for determination of paeoniflorin content in n-butanol extract of Radix Paeoniae Rubra by HPLC was established. The compound was separated by Diamonsil Cx8 column （4.6mm×250mm,5μm）with methanol（A）-ultra-pure water （0.2% acetic acid）（B）（30 ×70,V/V） as mobile phase at a flow rate of 1mL·min-1, and column temperature was 30℃, detection wavelength was 230 nm and injection volume was 10μL. The average content of paeoniflorin in n- butanol extract of Radix Paeoniae Rubra was 1.52%. The method for content determination is simple and accurate, and can be applied to quality control of the content of paeoniflorin. N-butanol can be used for purification of paeoniflorin.

关 键 词：赤芍 芍药苷 正丁醇 液液萃取 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]